The Induction of Long-Term Potentiation by Medial Septum Activation under Urethane Anesthesia Can Alter Gene Expression in the Hippocampus
Abstract
:1. Introduction
2. Results
2.1. Loss of Cholinergic Neurons in the Medial Septum Does Not Lead to Transcriptomic Changes in the Dorsal Hippocampus under Basal Conditions
2.2. Degeneration of Cholinergic Neurons in the dMSA Prevents Expression of Some Early Genes after the Induction of Long-Term Potentiation
2.3. Inhibition of the M1 Muscarinic Receptors Alters the fEPSP Characteristics but Does Not Affect the Expression of Early Genes
2.4. Inhibition of M1 Muscarinic Receptors Prevents LTP Induction and Induces Selective Suppression of the cyr61 Gene in the Dorsal Hippocampus
3. Discussion
4. Materials and Methods
4.1. 192IgG-Saporin Injection
4.2. RNAseq
4.3. RNA Isolation and Reverse Transcription
4.4. qPCR
4.5. Electrophysiology Recording and Drug Injection
4.6. Immunohistochemistry
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Dobryakova, Y.V.; Gerasimov, K.; Spivak, Y.S.; Korotkova, T.; Koryagina, A.; Deryabina, A.; Markevich, V.A.; Bolshakov, A.P. The Induction of Long-Term Potentiation by Medial Septum Activation under Urethane Anesthesia Can Alter Gene Expression in the Hippocampus. Int. J. Mol. Sci. 2023, 24, 12970. https://doi.org/10.3390/ijms241612970
Dobryakova YV, Gerasimov K, Spivak YS, Korotkova T, Koryagina A, Deryabina A, Markevich VA, Bolshakov AP. The Induction of Long-Term Potentiation by Medial Septum Activation under Urethane Anesthesia Can Alter Gene Expression in the Hippocampus. International Journal of Molecular Sciences. 2023; 24(16):12970. https://doi.org/10.3390/ijms241612970
Chicago/Turabian StyleDobryakova, Yulia V., Konstantin Gerasimov, Yulia S. Spivak, Tinna Korotkova, Alena Koryagina, Angelina Deryabina, Vladimir A. Markevich, and Alexey P. Bolshakov. 2023. "The Induction of Long-Term Potentiation by Medial Septum Activation under Urethane Anesthesia Can Alter Gene Expression in the Hippocampus" International Journal of Molecular Sciences 24, no. 16: 12970. https://doi.org/10.3390/ijms241612970