Properties of Plant Virus Protein Encoded by the 5′-Proximal Gene of Tetra-Cistron Movement Block
Abstract
:1. Introduction
2. Results
2.1. RNA-Binding Properties of vDRB
2.2. Subcellular Localization of vDRB
2.3. Suppression of RNA Silencing by vDRB in Non-Viral Experimental Systems
2.4. Suppression of RNA Silencing by vDRB in TCV-Based Assay
2.5. Influence of vDRB on PVX Infection
3. Discussion
4. Materials and Methods
4.1. Recombinant Constructs
4.2. Protein Expression in E. coli
4.3. Gel-Shift Experiments
4.4. Plant Material
4.5. Plant Agroinfiltration and Inoculation
4.6. Western Blot Analysis
4.7. Quantitative PCR
4.8. Microscopy and Virus Movement Visualization
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chergintsev, D.A.; Solovieva, A.D.; Atabekova, A.K.; Lezzhov, A.A.; Golyshev, S.A.; Morozov, S.Y.; Solovyev, A.G. Properties of Plant Virus Protein Encoded by the 5′-Proximal Gene of Tetra-Cistron Movement Block. Int. J. Mol. Sci. 2023, 24, 14144. https://doi.org/10.3390/ijms241814144
Chergintsev DA, Solovieva AD, Atabekova AK, Lezzhov AA, Golyshev SA, Morozov SY, Solovyev AG. Properties of Plant Virus Protein Encoded by the 5′-Proximal Gene of Tetra-Cistron Movement Block. International Journal of Molecular Sciences. 2023; 24(18):14144. https://doi.org/10.3390/ijms241814144
Chicago/Turabian StyleChergintsev, Denis A., Anna D. Solovieva, Anastasia K. Atabekova, Alexander A. Lezzhov, Sergei A. Golyshev, Sergey Y. Morozov, and Andrey G. Solovyev. 2023. "Properties of Plant Virus Protein Encoded by the 5′-Proximal Gene of Tetra-Cistron Movement Block" International Journal of Molecular Sciences 24, no. 18: 14144. https://doi.org/10.3390/ijms241814144