Genome-Wide Analysis of Cation/Proton Antiporter Family in Soybean (Glycine max) and Functional Analysis of GmCHX20a on Salt Response
Abstract
:1. Introduction
2. Results
2.1. Identification and Classification of CPA Genes in Soybean
2.2. Gene Structure and Conserved Domain Analysis
2.3. Chromosomal Locations and Expansion Pattern of GmCPAs
2.4. cis-Elements Analysis in the Promoter of GmCPAs
2.5. Expression Analysis of GmCPAs in Different Tissues
2.6. Expression Profiles of GmCPAs under Salt Stress and Osmotic Stress
2.7. Ectopic Expression of GmCHX20a Increased Salt Sensitivity in Arabidopsis
3. Discussion
4. Materials and Methods
4.1. Identification and Characteristics of CPA Genes in Soybean
4.2. Phylogenetic Analysis
4.3. Analysis of Gene Structures and Conserved Domains
4.4. Chromosome Localization and Gene Duplication Analysis
4.5. Analysis of cis-Acting Regulatory Elements in Promoter
4.6. Digital Expression Analysis of GmCPAs in Different Tissues or under Abiotic Stress
4.7. Gene Expression Analysis of GmCPAs under Salt or Osmotic Treatments
4.8. Salt Tolerance Assay on the GmCHX20a-Overexpressing Arabidopsis thaliana Plants
4.9. Subcellular Localization by Transient Expression in Onion Epidermis
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jia, Q.; Song, J.; Zheng, C.; Fu, J.; Qin, B.; Zhang, Y.; Liu, Z.; Jia, K.; Liang, K.; Lin, W.; et al. Genome-Wide Analysis of Cation/Proton Antiporter Family in Soybean (Glycine max) and Functional Analysis of GmCHX20a on Salt Response. Int. J. Mol. Sci. 2023, 24, 16560. https://doi.org/10.3390/ijms242316560
Jia Q, Song J, Zheng C, Fu J, Qin B, Zhang Y, Liu Z, Jia K, Liang K, Lin W, et al. Genome-Wide Analysis of Cation/Proton Antiporter Family in Soybean (Glycine max) and Functional Analysis of GmCHX20a on Salt Response. International Journal of Molecular Sciences. 2023; 24(23):16560. https://doi.org/10.3390/ijms242316560
Chicago/Turabian StyleJia, Qi, Junliang Song, Chengwen Zheng, Jiahui Fu, Bin Qin, Yongqiang Zhang, Zhongjuan Liu, Kunzhi Jia, Kangjing Liang, Wenxiong Lin, and et al. 2023. "Genome-Wide Analysis of Cation/Proton Antiporter Family in Soybean (Glycine max) and Functional Analysis of GmCHX20a on Salt Response" International Journal of Molecular Sciences 24, no. 23: 16560. https://doi.org/10.3390/ijms242316560