Imbalanced IL-1B and IL-18 Expression in Sézary Syndrome
Abstract
:1. Introduction
2. Results
2.1. Expression of Inflammasome Components in the Skin of Patients with SS
2.2. High Levels of Serum IL-18 and IL-18BPa in Sézary Syndrome
2.3. Inflammasome Profile in PBMC from Sézary Patients
2.4. Inflammasome Profile in Lymph Nodes of Sézary Patients
3. Discussion
4. Materials and Methods
4.1. Human Subjects
4.2. PBMC and Cell Lines
4.3. RNA Extraction, Sequencing and Transcriptomic Analyses
4.4. Gene Expression by Real-Time Polymerase Chain Reaction
4.5. Immunohistochemistry
4.6. Determination of IL-18 and IL-18BPa
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Manfrere, K.C.G.; Torrealba, M.P.; Ferreira, F.M.; de Sousa, E.S.A.; Miyashiro, D.; Teixeira, F.M.E.; Custódio, R.W.A.; Nakaya, H.I.; Ramos, Y.A.L.; Sotto, M.N.; et al. Imbalanced IL-1B and IL-18 Expression in Sézary Syndrome. Int. J. Mol. Sci. 2023, 24, 4674. https://doi.org/10.3390/ijms24054674
Manfrere KCG, Torrealba MP, Ferreira FM, de Sousa ESA, Miyashiro D, Teixeira FME, Custódio RWA, Nakaya HI, Ramos YAL, Sotto MN, et al. Imbalanced IL-1B and IL-18 Expression in Sézary Syndrome. International Journal of Molecular Sciences. 2023; 24(5):4674. https://doi.org/10.3390/ijms24054674
Chicago/Turabian StyleManfrere, Kelly Cristina Gomes, Marina Passos Torrealba, Frederico Moraes Ferreira, Emanuella Sarmento Alho de Sousa, Denis Miyashiro, Franciane Mouradian Emidio Teixeira, Ricardo Wesley Alberca Custódio, Helder I. Nakaya, Yasmin Alefe Leuzzi Ramos, Mirian Nacagami Sotto, and et al. 2023. "Imbalanced IL-1B and IL-18 Expression in Sézary Syndrome" International Journal of Molecular Sciences 24, no. 5: 4674. https://doi.org/10.3390/ijms24054674