Leuconostoc Citreum Inhibits Adipogenesis and Lipogenesis by Inhibiting p38 MAPK/Erk 44/42 and Stimulating AMPKα Signaling Pathways
Abstract
:1. Introduction
2. Results
2.1. Impact of Cell Free Supernatant of L. citreum (LSC) on Cell Viability
2.2. LSC Treatment Reduces Fat Deposition in 3T3-L1 Cells
2.3. LSC Downregulates Differentiation and Fatty Acid Synthesis Associated Proteins
2.4. LSC Competes with Rosiglitazone (RGZ)-Induced Lipid Accumulation and PPAR-γ Expressions
2.5. LSC Inhibits Differentiation and Fat Deposition through p38MAPK and Erk1/2 Signaling Pathways
2.6. LSC Induces Lipolysis by Activating AMPK-α in Differentiated Adipocytes
3. Discussion
4. Materials and Methods
4.1. Isolation and Characterization of Leuconostoc citreum
4.2. Production of Cell Free Supernatant of L. citreum (LSC) and Lyophilization
4.3. Cytotoxic Effects of LSC
4.4. Differentiation and Lipid Deposition Induction in 3T3-L1 Cells
4.5. Detection and Determination of Fat Deposition by Oil Red O (ORO) Staining
4.6. Proteins Extraction and Immunoblot Analysis
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Han, H.-S.; Soundharrajan, I.; Valan Arasu, M.; Kim, D.; Choi, K.-C. Leuconostoc Citreum Inhibits Adipogenesis and Lipogenesis by Inhibiting p38 MAPK/Erk 44/42 and Stimulating AMPKα Signaling Pathways. Int. J. Mol. Sci. 2023, 24, 7367. https://doi.org/10.3390/ijms24087367
Han H-S, Soundharrajan I, Valan Arasu M, Kim D, Choi K-C. Leuconostoc Citreum Inhibits Adipogenesis and Lipogenesis by Inhibiting p38 MAPK/Erk 44/42 and Stimulating AMPKα Signaling Pathways. International Journal of Molecular Sciences. 2023; 24(8):7367. https://doi.org/10.3390/ijms24087367
Chicago/Turabian StyleHan, Hyo-Shim, Ilavenil Soundharrajan, Mariadhas Valan Arasu, Dahye Kim, and Ki-Choon Choi. 2023. "Leuconostoc Citreum Inhibits Adipogenesis and Lipogenesis by Inhibiting p38 MAPK/Erk 44/42 and Stimulating AMPKα Signaling Pathways" International Journal of Molecular Sciences 24, no. 8: 7367. https://doi.org/10.3390/ijms24087367