Inhibition of BET Proteins Regulates Fcγ Receptor Function and Reduces Inflammation in Rheumatoid Arthritis
Abstract
:1. Introduction
2. Results and Discussionγ
2.1. BET Inhibition Reduces Fcγ Receptor Expression in Peripheral Blood Monocytes
2.2. BET Inhibition Downregulates Fcγ Receptor-Mediated Signaling
2.3. BET Inhibition Attenuates Monocyte FcγR Function
2.4. BET Inhibition Regulates Fc𝛾 Receptor Expression and Function in Monocytes from Rheumatoid Arthritis Patients
2.5. BET Inhibition Reduces Footpad Inflammation and FcγR Expression In Vivo
3. Materials and Methods
3.1. Peripheral Blood Monocyte Isolation
3.2. Cell Culture Treatments
3.3. Immunoblotting
3.4. Phagocytosis
3.5. Rosetting
3.6. Real-Time Polymerase Chain Reaction (qPCR)
3.7. Enzyme Linked Immunosorbence Assay (ELISA)
3.8. Fc𝛾 Receptor Flow Cytometry
3.9. Collagen-Induced Arthritis Mouse Model
3.10. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Shankar, D.; Merchand-Reyes, G.; Buteyn, N.J.; Santhanam, R.; Fang, H.; Kumar, K.; Mo, X.; Ganesan, L.P.; Jarjour, W.; Butchar, J.P.; et al. Inhibition of BET Proteins Regulates Fcγ Receptor Function and Reduces Inflammation in Rheumatoid Arthritis. Int. J. Mol. Sci. 2023, 24, 7623. https://doi.org/10.3390/ijms24087623
Shankar D, Merchand-Reyes G, Buteyn NJ, Santhanam R, Fang H, Kumar K, Mo X, Ganesan LP, Jarjour W, Butchar JP, et al. Inhibition of BET Proteins Regulates Fcγ Receptor Function and Reduces Inflammation in Rheumatoid Arthritis. International Journal of Molecular Sciences. 2023; 24(8):7623. https://doi.org/10.3390/ijms24087623
Chicago/Turabian StyleShankar, Divya, Giovanna Merchand-Reyes, Nathaniel J. Buteyn, Ramasamy Santhanam, Huiqing Fang, Krishan Kumar, Xiaokui Mo, Latha P. Ganesan, Wael Jarjour, Jonathan P. Butchar, and et al. 2023. "Inhibition of BET Proteins Regulates Fcγ Receptor Function and Reduces Inflammation in Rheumatoid Arthritis" International Journal of Molecular Sciences 24, no. 8: 7623. https://doi.org/10.3390/ijms24087623