KCNG4 Genetic Variant Linked to Migraine Prevents Expression of KCNB1
Abstract
:1. Introduction
2. Results
2.1. Kv2.1/Kv6.4-L360P Complexes Are Strongly Translated and Assembled
2.2. Kv2.1/Kv6.4-L360P Complexes Are Not Trafficked to the Plasma Membrane
3. Discussion
4. Materials and Methods
4.1. Pulse Protocols and Data Analysis
4.2. Expression of Kv2/Kv6 in Xenopus Oocytes
4.3. Immunoblotting of Kv2.1 and Kv6.4
4.3.1. Xenopus Oocytes
4.3.2. Mammalian Cell Lines
4.3.3. Cell Surface Biotinylation Assay
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Conductance | V50 (mV) | Slope (mV) | Apparent Charge | N | |
Kv2.1 | 0.1 ± 0.8 | 10.9 ± 0.7 | 2.37 ± 0.14 | 6 | |
Kv2.1/Kv6.4 | −9.1 ± 0.6 | 15.9 ± 0.5 | 1.63 ± 0.05 | 18 | |
Kv2.1/Kv6.4-L360P | −0.4 ± 0.6 | 10.7 ± 0.6 | 2.41 ± 0.12 | 9 | |
Inactivation | V50(mV) | Slope (mV) | Apparent Charge | Maximum Inactivation | N |
Kv2.1 | −23.8 ± 0.4 | −6.0 ± 0.3 | −4.19 ± 0.21 | 0.58 ± 0.02 | 9 |
Kv2.1/Kv6.4 | −60.7 ± 0.6 | −8.4 ± 0.4 | −3.07 ± 0.14 | 0.55 ± 0.02 | 11 |
Kv2.1/Kv6.4-L360P | −24.7 ± 0.6 | −6.1 ± 0.5 | −4.24 ± 0.33 | 0.61 ± 0.02 | 6 |
Incubation Time | Constructs | Expression (µA) | Ratio to Kv2.1 (%) | |
---|---|---|---|---|
18–24 h | Kv2.1 | 33.2 ± 30.5 | - | |
Kv2.1/Kv6.4 | 2.9 ± 3.6 | 8.7 | }p < 0.03 | |
Kv2.1/Kv6.4-L360P | 0.2 ± 0.3 | 0.6 | ||
36–48 h | Kv2.1 | 53.3 ± 28.5 | - | |
Kv2.1/Kv6.4 | 14.6 ± 6.9 | 27.4 | }p < 10−9 | |
Kv2.1/Kv6.4-L360P | 0.2 ± 0.3 | 0.4 | ||
66–72 h | Kv2.1 | 69.2 ± 28.8 | - | |
Kv2.1/Kv6.4 | 22.3 ± 18.4 | 32.2 | }p < 0.0001 | |
Kv2.1/Kv6.4-L360P | 2.9 ± 4.0 | 4.2 |
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Lacroix, G.; Bhat, S.; Shafia, Z.; Blunck, R. KCNG4 Genetic Variant Linked to Migraine Prevents Expression of KCNB1. Int. J. Mol. Sci. 2024, 25, 8960. https://doi.org/10.3390/ijms25168960
Lacroix G, Bhat S, Shafia Z, Blunck R. KCNG4 Genetic Variant Linked to Migraine Prevents Expression of KCNB1. International Journal of Molecular Sciences. 2024; 25(16):8960. https://doi.org/10.3390/ijms25168960
Chicago/Turabian StyleLacroix, Gabriel, Shreyas Bhat, Zerghona Shafia, and Rikard Blunck. 2024. "KCNG4 Genetic Variant Linked to Migraine Prevents Expression of KCNB1" International Journal of Molecular Sciences 25, no. 16: 8960. https://doi.org/10.3390/ijms25168960