Transcriptomic and Physiological Studies Unveil that Brassinolide Maintains the Balance of Maize’s Multiple Metabolisms under Low-Temperature Stress
Abstract
:1. Introduction
2. Results
2.1. Effects of Exogenous EBR on Physiological Metabolisms under LT Stress
2.2. Pearson Correlation and Hierarchical Cluster Analyses
2.3. Evaluation of the RNA-Seq Dataset
2.4. Defining DEGs
2.5. Functional Annotation DEGs
2.6. DEGs Involved in ROS Homeostasis
2.7. DEGs Involved in Plant Hormone Signal Transduction
2.8. DEGs Involved in the MAPK Cascade
2.9. WGCNA Analysis and Gene Co-Expression Network Construction
2.10. Verification of RNA-Seq Data by Quantitative Real-Time PCR (qRT-PCR)
3. Discussion
4. Materials and Methods
4.1. Experimental Materials and Treatments
4.2. Physiological Metabolism Measurements
4.3. Statistical Analyses
4.4. RNA-Seq and DEG Identification
4.5. WGCNA Analysis and Network Construction
4.6. qRT-PCR Validation
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhao, X.; He, F.; Qi, G.; Sun, S.; Shi, Z.; Niu, Y.; Wu, Z. Transcriptomic and Physiological Studies Unveil that Brassinolide Maintains the Balance of Maize’s Multiple Metabolisms under Low-Temperature Stress. Int. J. Mol. Sci. 2024, 25, 9396. https://doi.org/10.3390/ijms25179396
Zhao X, He F, Qi G, Sun S, Shi Z, Niu Y, Wu Z. Transcriptomic and Physiological Studies Unveil that Brassinolide Maintains the Balance of Maize’s Multiple Metabolisms under Low-Temperature Stress. International Journal of Molecular Sciences. 2024; 25(17):9396. https://doi.org/10.3390/ijms25179396
Chicago/Turabian StyleZhao, Xiaoqiang, Fuqiang He, Guoxiang Qi, Siqi Sun, Zhenzhen Shi, Yining Niu, and Zefeng Wu. 2024. "Transcriptomic and Physiological Studies Unveil that Brassinolide Maintains the Balance of Maize’s Multiple Metabolisms under Low-Temperature Stress" International Journal of Molecular Sciences 25, no. 17: 9396. https://doi.org/10.3390/ijms25179396