L-Glutamate Regulates Npy via the mGluR4-Ca2+-ERK1/2 Signaling Pathway in Mandarin Fish (Siniperca chuatsi)
Abstract
:1. Introduction
2. Results
2.1. Synteny, Sequence Alignment, and Phylogenetic Analysis of mGluR4 Genes in Mandarin Fish
2.2. L-Glutamate Regulates Npy via mGluR4 in Mandarin Fish Brain Cells
2.3. Ca2+ Involvement in L-Glutamate Regulating Npy through mGluR4 in Mandarin Fish Brain Cells
2.4. ERK1/2 Involvement in L-Glutamate Regulating Npy through mGluR4 in Mandarin Fish Brain Cells
3. Discussion
4. Materials and Methods
4.1. mGluR4 Sequence Analysis
4.2. Fish and Sample Collection
4.3. Mandarin Fish Brain Cell Culture and Treatment
4.4. Cell Counting Kit-8 (CCK-8)
4.5. Cloning mGluR4 Gene Sequences of Mandarin Fish
4.6. mGluR4 Functional Expression
4.7. Calcium Assays
4.8. RT-PCR
4.9. Western Blot
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Species | Siniperca chuatsi (%) |
---|---|
Homo sapiens | 79 |
Mus musculus | 79 |
Danio rerio | 85 |
Oryzias latipes | 84 |
Morone saxatilis | 96 |
Ctenopharyngodon idella | 86 |
Gene Name Primer | Sequence of Primer (5′ to 3′) | Tm (°C) |
---|---|---|
mGluR4-pcDNA3.1 | F TAGTCCAGTGTGGTGGAATTC ATGACTCTACGAGGAGGTTTTGA R GGTTTAAACGGGCCCTCTAGA TTAGATGGCATGGTTACTGTAGC | 58 |
Gene Name (%) | Sequence of Primer (5′ to 3′) | Tm (°C) | Amplification Efficiency |
---|---|---|---|
rpl13a | F TATCCCCCCACCCTATGACA R ACGCCCAAGGAGAGCGAACT | 58.0 | 104.8 |
mGluR4 | F GAGTGGTGGGAGTGATTGGTG R GGGAGAAGAAGTCATAGCGGG | 58.0 | 96.4 |
npy | F GTTGAAGGAAAGCACAGACA R GCTCATAGAGGTAAAAGGGG | 60.0 | 92.3 |
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Duan, J.; Wang, Q.; He, S.; Liang, X.-F.; Ding, L. L-Glutamate Regulates Npy via the mGluR4-Ca2+-ERK1/2 Signaling Pathway in Mandarin Fish (Siniperca chuatsi). Int. J. Mol. Sci. 2024, 25, 10035. https://doi.org/10.3390/ijms251810035
Duan J, Wang Q, He S, Liang X-F, Ding L. L-Glutamate Regulates Npy via the mGluR4-Ca2+-ERK1/2 Signaling Pathway in Mandarin Fish (Siniperca chuatsi). International Journal of Molecular Sciences. 2024; 25(18):10035. https://doi.org/10.3390/ijms251810035
Chicago/Turabian StyleDuan, Jiahui, Qiuling Wang, Shan He, Xu-Fang Liang, and Liyun Ding. 2024. "L-Glutamate Regulates Npy via the mGluR4-Ca2+-ERK1/2 Signaling Pathway in Mandarin Fish (Siniperca chuatsi)" International Journal of Molecular Sciences 25, no. 18: 10035. https://doi.org/10.3390/ijms251810035