Knockdown of Adenosine 5′-Triphosphate-Dependent Caseinolytic Protease Proteolytic Subunit 6 Enhances Aluminum Tolerance in Peanut Plants (Arachis hypogea L.)
Abstract
:1. Introduction
2. Results
2.1. Phylogenetic Analysis of AhClpP6
2.2. AhClpP6 Expression Increased with Al3+ Stress in Peanut Plants
2.3. Sequencing and Identification of Differentially Expressed Genes
2.4. GO and KEGG Annotation of DEGs
2.5. AhClpP6 Positively Regulates Al3+ Tolerance by Modulating Photosynthesis
2.6. AhClpP6 Positively Regulates Chloroplast Integrity
3. Discussion
4. Materials and Methods
4.1. Peanut (Arachis hypogea L.) Cultivation and Al3+ Treatment
4.2. Multiple Sequence Alignments and Phylogenetic Analysis
4.3. RT-qPCR Analysis
4.4. Virus-Induced Gene Silencing (VIGS)
4.5. RNA-Seq and Transcriptome Analysis
4.6. Al3+ Tolerance Assay
4.7. Photosynthesis and Chl Fluorescence Measurements
4.8. Transmission Electron Microscopy Observation of Cell Ultrastructure
4.9. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Shi, Y.; Zhang, D.; Liang, R.; Xiao, D.; Wang, A.; He, L.; Zhan, J. Knockdown of Adenosine 5′-Triphosphate-Dependent Caseinolytic Protease Proteolytic Subunit 6 Enhances Aluminum Tolerance in Peanut Plants (Arachis hypogea L.). Int. J. Mol. Sci. 2024, 25, 10416. https://doi.org/10.3390/ijms251910416
Shi Y, Zhang D, Liang R, Xiao D, Wang A, He L, Zhan J. Knockdown of Adenosine 5′-Triphosphate-Dependent Caseinolytic Protease Proteolytic Subunit 6 Enhances Aluminum Tolerance in Peanut Plants (Arachis hypogea L.). International Journal of Molecular Sciences. 2024; 25(19):10416. https://doi.org/10.3390/ijms251910416
Chicago/Turabian StyleShi, Yusun, Dayue Zhang, Ronghua Liang, Dong Xiao, Aiqin Wang, Longfei He, and Jie Zhan. 2024. "Knockdown of Adenosine 5′-Triphosphate-Dependent Caseinolytic Protease Proteolytic Subunit 6 Enhances Aluminum Tolerance in Peanut Plants (Arachis hypogea L.)" International Journal of Molecular Sciences 25, no. 19: 10416. https://doi.org/10.3390/ijms251910416