Genome-Wide Characterization and Functional Validation of the ACS Gene Family in the Chestnut Reveals Its Regulatory Role in Ovule Development
Abstract
:1. Introduction
2. Results
2.1. Genome-Wide Analysis of the Chestnut ACS Gene (CmACS) Family
2.2. Multiple-Sequence Alignment and Phylogenetic Analysis of the CmACS Gene Family
2.3. Structural Characterization of CmACS Proteins
2.4. Analysis of Cis-Acting Elements in CmACS Promoters
2.5. Chromosome Localization and Covariance Analysis of CmACSs
2.6. Tissue Expression Pattern Analysis of CmACSs
2.7. Expression Analysis of CmACS Genes during Ovule Development
2.8. Subcellular Localization
2.9. Genetic Transformation of Chestnut Healing Tissues
2.10. Genetic Transformation of Micro S. lycoperisicum
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Identification and Protein Structure Analysis of the CmACSs
4.3. Analysis of Cis-Acting Elements of ACS Promoters
4.4. Multiple-Sequence Comparison and Construction of the Phylogenetic Evolutionary Tree of CmACSs
4.5. Chromosome Location and Collinearity Analysis
4.6. Analysis of the Expression of CmACSs in Different Tissues
4.7. Expression Analysis of CmACSs during Ovule Development
4.8. Construction of CmACS7 Gene Overexpression and Interference Vectors
4.9. Subcellular Localization
4.10. Genetic Transformation of Chestnut Healing Tissues
4.11. Transformation of S. lycoperisicum by A. tumefaciens Containing CmACS7
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Gene ID | Subcellular Localization | CDS Length/bp | No. of Exons | Protein Characteristics | Signal Peptide Sites | |||
---|---|---|---|---|---|---|---|---|---|
Amino Acid Length/aa | Instability Coefficient | Molecular Weight/kDa | Isoelectric Point | ||||||
CmACS1 | Cm01G01961 | Cytosol | 1459 | 2 | 485 | 50.07 | 54.60 | 6.06 | No |
CmACS2 | Cm03G01038 | Cytosol | 1435 | 4 | 477 | 45.94 | 53.82 | 8.14 | No |
CmACS3 | Cm08G00844 | Nucleus | 1414 | 6 | 470 | 41.07 | 53.25 | 6.90 | No |
CmACS4 | Cm09G01545 | Cytosol | 1417 | 4 | 471 | 43.91 | 53.14 | 5.54 | No |
CmACS5 | Cm12G01476 | Cytosol | 1477 | 4 | 491 | 43.43 | 55.17 | 6.74 | No |
CmACS6 | Cm12G01477 | Cytosol | 1477 | 4 | 491 | 43.43 | 55.17 | 6.74 | No |
CmACS7 | Cm12G01910 | Nucleus | 1607 | 5 | 535 | 50.79 | 59.25 | 7.02 | No |
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Cui, Y.; Ji, X.; Yu, W.; Liu, Y.; Bai, Q.; Su, S. Genome-Wide Characterization and Functional Validation of the ACS Gene Family in the Chestnut Reveals Its Regulatory Role in Ovule Development. Int. J. Mol. Sci. 2024, 25, 4454. https://doi.org/10.3390/ijms25084454
Cui Y, Ji X, Yu W, Liu Y, Bai Q, Su S. Genome-Wide Characterization and Functional Validation of the ACS Gene Family in the Chestnut Reveals Its Regulatory Role in Ovule Development. International Journal of Molecular Sciences. 2024; 25(8):4454. https://doi.org/10.3390/ijms25084454
Chicago/Turabian StyleCui, Yanhong, Xingzhou Ji, Wenjie Yu, Yang Liu, Qian Bai, and Shuchai Su. 2024. "Genome-Wide Characterization and Functional Validation of the ACS Gene Family in the Chestnut Reveals Its Regulatory Role in Ovule Development" International Journal of Molecular Sciences 25, no. 8: 4454. https://doi.org/10.3390/ijms25084454