CgMFS1, a Major Facilitator Superfamily Transporter, Is Required for Sugar Transport, Oxidative Stress Resistance, and Pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Strains and Culture Conditions
2.2. Bioinformatics Analysis
2.3. Construction of Knock-Out and Complementation Mutants
2.4. Colony Growth Assay
2.5. Stresses and Sensitivity Tests
2.6. Quantitative RT-PCR Analysis
2.7. Pathogenicity Assay
2.8. Appressorium Formation Assay
2.9. Statistical Analysis
3. Results
3.1. Identification of CgMFS1
3.2. Generation of Knock-Out and Complementation Mutants
3.3. CgMFS1 Is Required for Carbon Utilization
3.4. CgMFS1 Is Required for Tolerance to Oxidative and Fungicide Stresses
3.5. CgMFS1 Plays a Role in Fungal Pathogenicity
3.6. CgMFS1 Is Not Required for Conidial Germination and Appressorium Formation
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Conflicts of Interest
References
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Liu, N.; Wang, Q.; He, C.; An, B. CgMFS1, a Major Facilitator Superfamily Transporter, Is Required for Sugar Transport, Oxidative Stress Resistance, and Pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis. Curr. Issues Mol. Biol. 2021, 43, 1548-1557. https://doi.org/10.3390/cimb43030109
Liu N, Wang Q, He C, An B. CgMFS1, a Major Facilitator Superfamily Transporter, Is Required for Sugar Transport, Oxidative Stress Resistance, and Pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis. Current Issues in Molecular Biology. 2021; 43(3):1548-1557. https://doi.org/10.3390/cimb43030109
Chicago/Turabian StyleLiu, Na, Qiannan Wang, Chaozu He, and Bang An. 2021. "CgMFS1, a Major Facilitator Superfamily Transporter, Is Required for Sugar Transport, Oxidative Stress Resistance, and Pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis" Current Issues in Molecular Biology 43, no. 3: 1548-1557. https://doi.org/10.3390/cimb43030109