Surface Characterisation of Human Serum Albumin Layers on Activated Ti6Al4V
Abstract
:1. Introduction
2. Materials and Methods
3. Results
3.1. Protein Adsorption and Structural Reorganisation
3.2. Surface Hydrophobicity
3.3. Electric Potential of Interaction
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ion Fragments | m/z | Ion Fragments | m/z |
---|---|---|---|
CH4N+ (Glycine, Gly) | 30.036 | C4H5N2+ (Histidine, His) | 81.048 |
C2H6N+ (Alanine, Ala) | 44.052 | C4H6NO+ (Glutaic Acid, Glu) | 84.051 |
Ti+ | 47.948 | C5H12N+ (Leucine, Leu) | 86.107 |
C4H5O+ (Threonine, Thr) | 69.038 | C3H7N2O+ (Asparagine, Asn) | 87.067 |
C4H8N+ (Proline, Pro) | 70.072 | C3H6NO2+ (Aspartic Acid, Asp) | 88.047 |
C4H10N+ (Valine, Val) | 72.087 | C4H10N3+ (Arginine, Arg) | 100.092 |
Sample | θW (°) | θD (°) | ∆GW (mJ/m2) | |||
---|---|---|---|---|---|---|
TiAlV | 74 ± 5 | 54 ± 3 | 32 ± 2 | 8 ± 3 | 40 ± 5 | −53 ± 9 |
TiAlV + HSA | 62 ± 8 | 48 ± 9 | 35 ± 5 | 13 ± 7 | 48 ± 11 | −38 ± 17 |
TiAlV UV 2h | 46 ± 5 | 31 ± 3 | 44 ± 1 | 19 ± 3 | 62 ± 5 | −22 ± 6 |
TiAlV UV 2h + HSA | 51 ± 21 | 48 ± 7 | 35 ± 4 | 20 ± 15 | 55 ± 19 | −27 ± 27 |
TiAlV UV 15h | 0 | 24 ± 2 | 47 ± 1 | 33 ± 1 | 79 ± 1 | 0 ± 1 |
TiAlV UV 15h + HSA | 72 ± 2 | 48 ± 4 | 35 ± 2 | 8 ± 2 | 43 ± 4 | −50 ± 6 |
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Hierro-Oliva, M.; Gallardo-Moreno, A.M.; González-Martín, M.L. Surface Characterisation of Human Serum Albumin Layers on Activated Ti6Al4V. Materials 2021, 14, 7416. https://doi.org/10.3390/ma14237416
Hierro-Oliva M, Gallardo-Moreno AM, González-Martín ML. Surface Characterisation of Human Serum Albumin Layers on Activated Ti6Al4V. Materials. 2021; 14(23):7416. https://doi.org/10.3390/ma14237416
Chicago/Turabian StyleHierro-Oliva, Margarita, Amparo M. Gallardo-Moreno, and María Luisa González-Martín. 2021. "Surface Characterisation of Human Serum Albumin Layers on Activated Ti6Al4V" Materials 14, no. 23: 7416. https://doi.org/10.3390/ma14237416