Changes in Seed Germination Ability, Lipid Peroxidation and Antioxidant Enzyme Activities of Ginkgo biloba Seed during Desiccation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Experimental Designs
2.2. Measurements
2.2.1. Determination of Moisture Content (MC)
2.2.2. Germination Test
2.2.3. Electrical Conductivity Assay
2.2.4. Determination of Anti-Oxidation System (SOD, POD Activity, and Malondialdehyde (MDA) Content)
2.3. Statistical Analysis
3. Results
3.1. Germination Changes and Electrical Conductivity
3.2. SOD, POD Activity, and MDA Contents
3.3. Correlation Analysis
4. Discussion
4.1. Changes of Germination in G. biloba Seeds during Desiccation
4.2. Changes of Electrical Conductivity in G. biloba Seeds during Desiccation
4.3. Changes of SOD and POD Activity in G. biloba Seeds during Desiccation
4.4. Changes of MDA Content in G. biloba Seeds during Desiccation
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Source | Sum of Squares | df | Mean Square | F | p | |
---|---|---|---|---|---|---|
Germination percentage | Between Groups | 2.359 | 7 | 0.337 | 116.831 | <0.001 |
Within Groups | 0.046 | 16 | 0.003 | |||
Total | 2.405 | 23 | ||||
Electrical conductivity | Between Groups | 0.269 | 7 | 0.038 | 39.305 | <0.001 |
Within Groups | 0.016 | 16 | 0.001 | |||
Total | 0.284 | 23 | ||||
SOD activity | Between Groups | 279,608.451 | 7 | 39,944.064 | 7.252 | 0.001 |
Within Groups | 88,130.424 | 16 | 5508.152 | |||
Total | 367,738.876 | 23 | ||||
POD activity | Between Groups | 553.311 | 7 | 79.044 | 15.173 | <0.001 |
Within Groups | 83.350 | 16 | 5.209 | |||
Total | 636.661 | 23 | ||||
MDA content | Between Groups | 996.525 | 7 | 142.361 | 3.085 | 0.029 |
Within Groups | 738.356 | 16 | 46.147 | |||
Total | 1734.880 | 23 |
Physiological Indicators | MC | SOD Activity | MDA Content | POD Activity | Electrical Conductivity | Germination Percentage |
---|---|---|---|---|---|---|
MC | 1 | |||||
SOD activity | 0.118 | 1 | ||||
MDA content | −0.619 * | −0.373 | 1 | |||
POD activity | −0.002 | 0.595 * | −0.065 | 1 | ||
Electrical conductivity | −0.745 * | 0.092 | 0.421 * | 0.439 * | 1 | |
Germination percentage | 0.910 * | −0.107 | −0.545 * | −0.364 | −0.864 * | 1 |
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Feng, J.; Shen, Y.; Shi, F.; Li, C. Changes in Seed Germination Ability, Lipid Peroxidation and Antioxidant Enzyme Activities of Ginkgo biloba Seed during Desiccation. Forests 2017, 8, 286. https://doi.org/10.3390/f8080286
Feng J, Shen Y, Shi F, Li C. Changes in Seed Germination Ability, Lipid Peroxidation and Antioxidant Enzyme Activities of Ginkgo biloba Seed during Desiccation. Forests. 2017; 8(8):286. https://doi.org/10.3390/f8080286
Chicago/Turabian StyleFeng, Jing, Yongbao Shen, Fenghou Shi, and Chengzhong Li. 2017. "Changes in Seed Germination Ability, Lipid Peroxidation and Antioxidant Enzyme Activities of Ginkgo biloba Seed during Desiccation" Forests 8, no. 8: 286. https://doi.org/10.3390/f8080286