Transcellular Transport of Heparin-coated Magnetic Iron Oxide Nanoparticles (Hep-MION) Under the Influence of an Applied Magnetic Field
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials
2.2. Synthesis of the Hep-MION
2.3. Physicochemical characterization of the Hep-MION
2.4. Transport of the Hep-MION across the polyester membrane
2.5. Transport of the Hep-MION across the cell monolayer
3. Results and Discussion
3.1. Physicochemical characterization of the Hep-MION nanoparticles
3.2. Stability of the Hep-MION nanoparticle dispersions
Buffer | Water | HBSS + 10% FBS | HBSS + 1% FBS | ||||||
---|---|---|---|---|---|---|---|---|---|
Incubation Time (h) | 0 h | 5 h | 24 h | 0 h | 5 h | 24 h | 0 h | 5 h | 24 h |
Average Size (nm) | 61.37 | 57.93 | 59.50 | 75.47 | 76.70 | 76.37 | 1053.27 | 3515.53 | 17659.43 |
S.D. (nm) | 6.07 | 9.10 | 6.38 | 6.31 | 7.33 | 7.46 | 66.82 | 260.23 | 1243.76 |
3.3. Transport of the Hep-MION across porous membranes
Concentration of Hep-MION | HBSS with 10% FBS | HBSS with 1% FBS | ||
---|---|---|---|---|
Magnet (-) | Magnet (+) | Magnet (-) | Magnet (+) | |
Peff (10-3 cm/sec) | Peff (10-3 cm/sec) | |||
0.206 mg/mL | 3.19 (0.425) | 7.21 (0.53) | 3.04 (0.449) | 6.77 (1.53) |
0.2575 mg/mL | 5.73 (0.272) | 8.47 (0.408) | 2.67 (0.544) | 6.67 (0.679) |
0.412 mg/mL | 6.72 (0.17) | 7.85 (0.849) | 2.45 (0.425) | 5.88 (0.736) |
3.4. Transport of the Hep-MION across cell monolayers promoted by a magnetic field
Peff (10-6 cm/sec) | dM/dt (10-7 mg/sec) | |||||||
---|---|---|---|---|---|---|---|---|
Concentration (mg/mL) | 0.2575 | 0.412 | 0.2575 | 0.412 | ||||
Magnet | - | + | - | + | - | + | - | + |
Average | 4.2 | 24 | 0.9 | 6.6 | 3.5 | 20 | 1.2 | 9 |
S.D. | 0.8 | 3.7 | 0.5 | 0.7 | 0.7 | 3.2 | 0.7 | 1.0 |
p-value | 5.81 × 10-3 | 3.94 × 10-4 | 5.81 × 10-3 | 3.94 × 10-4 |
3.5. Accumulation of Hep-MION on cell monolayers induced by a magnetic field
4. Conclusions
Acknowledgements
References and Notes
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Min, K.A.; Yu, F.; Yang, V.C.; Zhang, X.; Rosania, G.R. Transcellular Transport of Heparin-coated Magnetic Iron Oxide Nanoparticles (Hep-MION) Under the Influence of an Applied Magnetic Field. Pharmaceutics 2010, 2, 119-135. https://doi.org/10.3390/pharmaceutics2020119
Min KA, Yu F, Yang VC, Zhang X, Rosania GR. Transcellular Transport of Heparin-coated Magnetic Iron Oxide Nanoparticles (Hep-MION) Under the Influence of an Applied Magnetic Field. Pharmaceutics. 2010; 2(2):119-135. https://doi.org/10.3390/pharmaceutics2020119
Chicago/Turabian StyleMin, Kyoung Ah, Faquan Yu, Victor C. Yang, Xinyuan Zhang, and Gus R. Rosania. 2010. "Transcellular Transport of Heparin-coated Magnetic Iron Oxide Nanoparticles (Hep-MION) Under the Influence of an Applied Magnetic Field" Pharmaceutics 2, no. 2: 119-135. https://doi.org/10.3390/pharmaceutics2020119