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Volume 9
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Journal of Xenobiotics is published by MDPI from Volume 10 Issue 1 (2020). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.

J. Xenobiot., Volume 9, Issue 1 (May 2019) – 1 article

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Brief Report
Detection of Polystyrene Nanoplastics in Biological Tissues with a Fluorescent Molecular Rotor Probe
by François Gagné
J. Xenobiot. 2019, 9(1), 8147; https://doi.org/10.4081/xeno.2019.8147 (registering DOI) - 17 May 2019
Cited by 25 | Viewed by 2198
Abstract
The release of nanoplastics (NPs) from the weathering and degradation of plastics is an important environmental concern. Given their small sizes and their invasiveness in cells, methods for the detection of NPs in biological tissues are urgently needed. A simple fluorescence-based methodology for [...] Read more.
The release of nanoplastics (NPs) from the weathering and degradation of plastics is an important environmental concern. Given their small sizes and their invasiveness in cells, methods for the detection of NPs in biological tissues are urgently needed. A simple fluorescence-based methodology for the detection of polystyrene NPs in biological tissues is proposed. The commercially available molecular rotor probe 9-(dicyanovinyl)-julolidine (DCVJ) has the properties to detect changes in hydrophobicity and microviscosity and was used to detect NPs. Increasing concentrations of 50 and 100 nm NPs in water and in tissue extracts were mixed with the DCVJ probe and the emission spectra determined between 480–800 nm at 450 nm excitation. The data revealed that NPs induces a second emission peak at 620 nm that differed from the normal spectra of the biological extract at 500 nm. A significant linear relationship was obtained for NPs of both sizes (r = 0.98; p < 0.001) with a theoretical limit of detection of 65 ng/mL. A simple and rapid microplate spectrofluorometric method for the semi-quantitative detection of polystyrene NPs in biological tissues is thus presented. Full article
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