Effect of Docosahexaenoic Acid on Ca2+ Signaling Pathways in Cerulein-Treated Pancreatic Acinar Cells, Determined by RNA-Sequencing Analysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Line and Treatments
2.2. Preparation of Total RNA Extracts and Library Construction
2.3. RNA-Sequencing and Bioinformatics Analysis
2.4. Validation of RNA-Seq Profiles by Real-time Polymerase Chain Reaction (PCR)
2.5. Statistical Analysis
3. Results
3.1. DHA Inhibits Cerulein-Induced Changes in the Transcriptomic Profile of AR42J Cells
3.2. DHA Suppresses Cerulein-Induced Alteration in the Calcium Signaling Pathway, Determined by Functional Annotation and Pathway Analysis of DEGs
3.3. DHA Inhibits Expression of RyR2, IP3RI, Relb, and c-fos Genes in Cerulein-Treated AR42J Cells, Determined by Real-Time PCR
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Gene Name | None Mapped Read 1 | Cerulein Mapped Read 1 | DHA Mapped Read 1 | Cerulein+DHA Mapped Read 1 |
---|---|---|---|---|
Fosb | 49 | 242 | 55 | 113 |
Relb | 38 | 194 | 62 | 156 |
Pde2a | 997 | 2143 | 919 | 1402 |
Rras2 | 2058 | 4369 | 1837 | 3879 |
Npas4 | 10 | 210 | 7 | 112 |
Pnlip | 3165 | 9804 | 2821 | 6068 |
Pdzd2 | 35 | 124 | 47 | 55 |
Trpc4 | 44 | 117 | 32 | 62 |
Ankrd34a | 38 | 261 | 26 | 178 |
Dkk2 | 97 | 275 | 53 | 194 |
Nr4a2 | 77 | 363 | 60 | 204 |
Gad1 | 237 | 763 | 182 | 483 |
Itga4 | 214 | 500 | 112 | 280 |
F2 | 121 | 293 | 140 | 188 |
Mylk2 | 1651 | 4457 | 1293 | 2648 |
Nfs1 | 205 | 1109 | 156 | 67 |
Rims4 | 118 | 567 | 125 | 401 |
Chrna4 | 414 | 1059 | 381 | 803 |
Pdk4 | 81 | 287 | 43 | 198 |
Reg3b | 12 | 2873 | 2 | 892 |
Reg1a | 11,048 | 26,879 | 9104 | 12,476 |
Itpr1 | 2398 | 6178 | 2656 | 4547 |
Bid | 109 | 317 | 105 | 213 |
M6pr | 421 | 1352 | 426 | 485 |
C1s | 51 | 276 | 45 | 139 |
Gucy2c | 138 | 2830 | 65 | 1420 |
Akap2 | 54 | 151 | 110 | 102 |
Cela3b | 810 | 5573 | 915 | 3610 |
Lbh | 652 | 2070 | 572 | 1366 |
Dock4 | 99 | 331 | 135 | 198 |
Fos | 14 | 226 | 34 | 164 |
Tshr | 70 | 556 | 80 | 496 |
Moap1 | 372 | 832 | 444 | 684 |
Pros1 | 54 | 122 | 52 | 100 |
Timp3 | 102 | 260 | 116 | 146 |
Matn2 | 111 | 268 | 159 | 234 |
Abcd2 | 146 | 436 | 101 | 346 |
Rgl3 | 84 | 168 | 76 | 133 |
Chrnb4 | 157 | 382 | 81 | 312 |
Plcl1 | 738 | 1457 | 332 | 1357 |
Ihh | 3020 | 10,806 | 1511 | 6831 |
Per1 | 146 | 436 | 101 | 346 |
Tmem130 | 465 | 1162 | 550 | 899 |
Hpd | 24 | 139 | 24 | 88 |
Sgsm1 | 666 | 1706 | 560 | 1286 |
Igsf8 | 2961 | 9669 | 3829 | 7782 |
Kcnj9 | 119 | 290 | 115 | 209 |
Gabra2 | 361 | 922 | 209 | 558 |
Klf2 | 61 | 302 | 117 | 185 |
Pdlim3 | 574 | 1796 | 294 | 886 |
RyR2 | 32 | 154 | 32 | 96 |
Impad1 | 129 | 447 | 117 | 326 |
Egr1 | 138 | 752 | 286 | 478 |
Mapk4 | 645 | 1750 | 508 | 1098 |
Junb | 32 | 154 | 32 | 96 |
Col18a1 | 277 | 1286 | 243 | 818 |
Armcx1 | 80 | 203 | 17 | 102 |
Metabolic Pathways | Gad1, Hpd, Hpse, Impad1, Nfs1, Pnlip |
---|---|
Neuroactive ligand-receptor interaction | Chrna4, Chrnb4, F2, Gabra2, Tshr |
Oxytocin signaling pathway | Fos, Itpr1, Kcnj9, Mylk2, Ryr2 |
Apelin signaling pathway | Itpr1, Klf2, Mylk2, Rras2, Ryr2 |
Proteoglycans in cancer | Hpse, Ihh, Itpr1, Rras2, Timp3 |
Pancreatic secretion | Cela3b, Itpr1, Pnlip, Ryr2 |
Regulation of actin cytoskeleton | F2, Itga4, Mylk2, Rras2 |
cAMP signaling pathway | Fos, Rras2, Ryr2, Tshr |
MAPK signaling pathway | Fos, Nr4a1, Relb, Rras2 |
Calcium signaling pathway | Itpr1, Mylk2, Ryr2 |
cGMP-PKG signaling pathway | Itpr1, Mylk2, Pde2a |
Apoptosis | Bid, Fos, Itpr1 |
Autophagy | Itpr1, Rab7b, Rras2 |
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Kim, S.H.; Park, Y.; Lim, J.W.; Kim, H. Effect of Docosahexaenoic Acid on Ca2+ Signaling Pathways in Cerulein-Treated Pancreatic Acinar Cells, Determined by RNA-Sequencing Analysis. Nutrients 2019, 11, 1445. https://doi.org/10.3390/nu11071445
Kim SH, Park Y, Lim JW, Kim H. Effect of Docosahexaenoic Acid on Ca2+ Signaling Pathways in Cerulein-Treated Pancreatic Acinar Cells, Determined by RNA-Sequencing Analysis. Nutrients. 2019; 11(7):1445. https://doi.org/10.3390/nu11071445
Chicago/Turabian StyleKim, Suhn Hyung, Yeeun Park, Joo Weon Lim, and Hyeyoung Kim. 2019. "Effect of Docosahexaenoic Acid on Ca2+ Signaling Pathways in Cerulein-Treated Pancreatic Acinar Cells, Determined by RNA-Sequencing Analysis" Nutrients 11, no. 7: 1445. https://doi.org/10.3390/nu11071445