Suppression of DNCB-Induced Atopic Skin Lesions in Mice by Wikstroemia indica Extract
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Sample Preparation
2.2. Mouse Model
2.3. DNCB-Induced AD Model and W. indica Extract Treatment
2.4. Histological Analysis
2.5. Measurement of IgE and IL-4 Levels by ELISA
2.6. Measurement of Transepidermal Water Loss and Skin Hydration
2.7. Analysis of HPLC/MS
2.8. Statistical Analysis
3. Results
3.1. Effects of W. indica Extract on AD-Like Symptoms in the DNCB Hairless Mouse Model
3.2. Effects of W. indica on Epidermal Thickness in DNCB-Induced Atopic Mice
3.3. Effects of W. indica on Mast Cell Infiltration in DNCB-Induced Atopic Mice
3.4. Effects of W. indica Extract on Serum IgE and IL-4 Levels in DNCB-Induced Atopic Mice
3.5. Effects of W. indica Extract on Skin Barrier Function in DNCB-Induced Atopic Mice
3.6. The Phytochemical Characterization of W. indica Using the High-Performance Liquid Chromatography/Mass Spectrometry (HPLC/MS)
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Lee, S.-Y.; Park, N.-J.; Jegal, J.; Jo, B.-G.; Choi, S.; Lee, S.W.; Uddin, M.S.; Kim, S.-N.; Yang, M.H. Suppression of DNCB-Induced Atopic Skin Lesions in Mice by Wikstroemia indica Extract. Nutrients 2020, 12, 173. https://doi.org/10.3390/nu12010173
Lee S-Y, Park N-J, Jegal J, Jo B-G, Choi S, Lee SW, Uddin MS, Kim S-N, Yang MH. Suppression of DNCB-Induced Atopic Skin Lesions in Mice by Wikstroemia indica Extract. Nutrients. 2020; 12(1):173. https://doi.org/10.3390/nu12010173
Chicago/Turabian StyleLee, So-Yeon, No-June Park, Jonghwan Jegal, Beom-Geun Jo, Sangho Choi, Sang Woo Lee, Md. Salah Uddin, Su-Nam Kim, and Min Hye Yang. 2020. "Suppression of DNCB-Induced Atopic Skin Lesions in Mice by Wikstroemia indica Extract" Nutrients 12, no. 1: 173. https://doi.org/10.3390/nu12010173