The Oncogene Metadherin Interacts with the Known Splicing Proteins YTHDC1, Sam68 and T-STAR and Plays a Novel Role in Alternative mRNA Splicing
Abstract
:1. Introduction
2. Results
2.1. Metadherin Interacts with Splicing Protein Ythdc1
2.2. Sam68-Associated Protein T-Star is Androgen-Regulated and Overexpressed in Prostate Cancer
2.3. Metadherin Directly Interacts with Splicing Complex Proteins Sam68 and T-Star
2.4. Metadherin Attenuates the Effect of Ythdc1 on Cd44v5-Luc Splicing
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Plasmids
4.3. CD44 Minigene Assays
4.4. Yeast Two-Hybrid Assay
4.5. Cell lysates, Western Blotting and Immunoprecipitation
4.6. Confocal Microscopy
4.7. Tissue Microarray and Immunohistochemistry
4.8. Statistics and Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Luxton, H.J.; Simpson, B.S.; Mills, I.G.; Brindle, N.R.; Ahmed, Z.; Stavrinides, V.; Heavey, S.; Stamm, S.; Whitaker, H.C. The Oncogene Metadherin Interacts with the Known Splicing Proteins YTHDC1, Sam68 and T-STAR and Plays a Novel Role in Alternative mRNA Splicing. Cancers 2019, 11, 1233. https://doi.org/10.3390/cancers11091233
Luxton HJ, Simpson BS, Mills IG, Brindle NR, Ahmed Z, Stavrinides V, Heavey S, Stamm S, Whitaker HC. The Oncogene Metadherin Interacts with the Known Splicing Proteins YTHDC1, Sam68 and T-STAR and Plays a Novel Role in Alternative mRNA Splicing. Cancers. 2019; 11(9):1233. https://doi.org/10.3390/cancers11091233
Chicago/Turabian StyleLuxton, Hayley J., Benjamin S. Simpson, Ian G. Mills, Nicola R. Brindle, Zeba Ahmed, Vasilis Stavrinides, Susan Heavey, Stefan Stamm, and Hayley C. Whitaker. 2019. "The Oncogene Metadherin Interacts with the Known Splicing Proteins YTHDC1, Sam68 and T-STAR and Plays a Novel Role in Alternative mRNA Splicing" Cancers 11, no. 9: 1233. https://doi.org/10.3390/cancers11091233