Mitochondrial ROS Produced in Human Colon Carcinoma Associated with Cell Survival via Autophagy
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. The UQCRB-Overexpressing CRC Cell Line HCT116 Is Sensitized by A1938-Induced Cell Death
2.2. Autophagy Is Induced in Mutant UQCRB-Overexpressing Cells (MT)
2.3. The Effects of UQCRB Overexpression on Activation of TRPML1 Ca2+ Channels and TFEB via mROS
2.4. The Effect of A1938 on Increased Lysosome Activity in MT Cells
2.5. The Effect of A1938 on Autophagy Flux by Suppression of mROS Generation
2.6. HCT116 Is Sensitized by A1938-Induced Autophagy Regulation
3. Discussion
4. Materials and Methods
4.1. Compounds and Antibodies
4.2. Cell Culture
4.3. Immunoblotting
4.4. Immunocytochemistry
4.5. mROS Measurements
4.6. The mRFP-GFP-LC3B Plasmid Transfection
4.7. GCaMP3-ML1 Ca2+ Imaging
4.8. EGFP-TFEB Nuclear Translocation Assay
4.9. Fluorescence Staining
4.10. Cell Proliferation Assay
4.11. Cell Death Assay
4.12. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gwak, E.J.; Kim, D.; Hwang, H.-Y.; Kwon, H.J. Mitochondrial ROS Produced in Human Colon Carcinoma Associated with Cell Survival via Autophagy. Cancers 2022, 14, 1883. https://doi.org/10.3390/cancers14081883
Gwak EJ, Kim D, Hwang H-Y, Kwon HJ. Mitochondrial ROS Produced in Human Colon Carcinoma Associated with Cell Survival via Autophagy. Cancers. 2022; 14(8):1883. https://doi.org/10.3390/cancers14081883
Chicago/Turabian StyleGwak, Eun Ji, Dasol Kim, Hui-Yun Hwang, and Ho Jeong Kwon. 2022. "Mitochondrial ROS Produced in Human Colon Carcinoma Associated with Cell Survival via Autophagy" Cancers 14, no. 8: 1883. https://doi.org/10.3390/cancers14081883