QCM Biosensor Based on Polydopamine Surface for Real-Time Analysis of the Binding Kinetics of Protein-Protein Interactions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. PDA Coating
2.3. Contact Angle Measurement
2.4. X-ray Photoelectron Spectroscopy (XPS) Measurement
2.5. Protein Immobilization
2.6. QCM Analysis of Protein-Protein Interactions
2.7. Reuse of the Sensor Chip
3. Results and Discussion
3.1. PDA Coating
3.2. Protein Immobilization on the PDA-Coated Sensor Chip Surface
3.3. QCM Measurements of Protein-Protein Interaction
3.4. Kinetic and Affinity Studies
3.5. Reuse of the Sensor Chip for Knietic and Affinity Studies
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Wu, C.; Li, X.; Song, S.; Pei, Y.; Guo, L.; Pei, Z. QCM Biosensor Based on Polydopamine Surface for Real-Time Analysis of the Binding Kinetics of Protein-Protein Interactions. Polymers 2017, 9, 482. https://doi.org/10.3390/polym9100482
Wu C, Li X, Song S, Pei Y, Guo L, Pei Z. QCM Biosensor Based on Polydopamine Surface for Real-Time Analysis of the Binding Kinetics of Protein-Protein Interactions. Polymers. 2017; 9(10):482. https://doi.org/10.3390/polym9100482
Chicago/Turabian StyleWu, Chunli, Xueming Li, Siyu Song, Yuxin Pei, Lili Guo, and Zhichao Pei. 2017. "QCM Biosensor Based on Polydopamine Surface for Real-Time Analysis of the Binding Kinetics of Protein-Protein Interactions" Polymers 9, no. 10: 482. https://doi.org/10.3390/polym9100482