Characterization of Antagonist Potential of Selected Compost Bacterial Isolates (CBI) against Plant and Human Pathogens
Abstract
:1. Introduction
2. Materials and Methods
3. Results
3.1. Microbial Counts at Different Composting Times
3.2. pH and Biomass Variations
3.3. Antagonist Activity—Fungal Pathogens
3.4. Antagonist Activity—Pathogenic Bacteria
3.5. Physiological and Biochemical Testing
3.6. Raw Data Statistics of 16S Sequencing
3.7. Taxonomic Classification and Abundance Ratio (%)
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Phase 1 | ||||||||||
Soil | Pile A | Pile B | Pile C | Pile D | ISP 2 | NA | ISP 6 | ISP7 | PDA | |
Soil | 1.000 | |||||||||
Pile A | −0.250 | 1.000 | ||||||||
Pile B | −0.250 | −0.250 | 1.000 | |||||||
Pile C | −0.250 | −0.250 | −0.250 | 1.000 | ||||||
Pile D | −0.250 | −0.250 | −0.250 | −0.250 | 1.000 | |||||
ISP 2 | −0.332 | −0.336 | −0.332 | 0.956 | 0.044 | 1.000 | ||||
NA | −0.339 | −0.341 | −0.339 | 0.948 | 0.072 | 1.000 | 1.000 | |||
ISP 6 | −0.365 | −0.365 | −0.364 | 0.902 | 0.191 | 0.989 | 0.993 | 1.000 | ||
ISP7 | −0.396 | −0.398 | −0.397 | 0.413 | 0.779 | 0.661 | 0.681 | 0.765 | 1.000 | |
PDA | −0.343 | −0.343 | −0.342 | 0.087 | 0.943 | 0.374 | 0.400 | 0.507 | 0.943 | 1.000 |
Phase 2 | ||||||||||
Soil | Pile A | Pile B | Pile C | Pile D | ISP 2 | NA | ISP 6 | ISP7 | PDA | |
Soil | 1.000 | |||||||||
Pile A | −0.250 | 1.000 | ||||||||
Pile B | −0.250 | −0.250 | 1.000 | |||||||
Pile C | −0.250 | −0.250 | −0.250 | 1.000 | ||||||
Pile D | −0.250 | −0.250 | −0.250 | −0.250 | 1.000 | |||||
ISP 2 | −0.390 | 0.821 | 0.347 | −0.390 | −0.389 | 1.000 | ||||
NA | −0.421 | 0.178 | 0.903 | −0.268 | −0.393 | 0.704 | 1.000 | |||
ISP 6 | −0.406 | 0.940 | 0.062 | −0.200 | −0.397 | 0.947 | 0.482 | 1.000 | ||
ISP7 | −0.328 | 0.004 | 0.967 | −0.320 | −0.324 | 0.574 | 0.980 | 0.312 | 1.000 | |
PDA | −0.393 | 0.828 | 0.335 | −0.390 | −0.381 | 1.000 | 0.695 | 0.951 | 0.564 | 1.000 |
Phase 3 | ||||||||||
Soil | Pile A | Pile B | Pile C | Pile D | ISP 2 | NA | ISP 6 | ISP7 | PDA | |
Soil | 1.000 | |||||||||
Pile A | −0.250 | 1.000 | ||||||||
Pile B | −0.250 | −0.250 | 1.000 | |||||||
Pile C | −0.250 | −0.250 | −0.250 | 1.000 | ||||||
Pile D | −0.250 | −0.250 | −0.250 | −0.250 | 1.000 | |||||
ISP 2 | −0.291 | −0.168 | −0.265 | 0.996 | −0.272 | 1.000 | ||||
NA | −0.427 | −0.310 | 0.877 | 0.240 | −0.380 | 0.229 | 1.000 | |||
ISP 6 | −0.301 | −0.211 | −0.259 | 0.998 | −0.226 | 0.998 | 0.234 | 1.000 | ||
ISP7 | −0.475 | −0.225 | 0.796 | 0.362 | −0.458 | 0.360 | 0.985 | 0.359 | 1.000 | |
PDA | −0.298 | −0.293 | 0.979 | 0.998 | −0.298 | −0.115 | 0.940 | −0.108 | 0.876 | 1.000 |
Pathogenic Bacteria Inhibition Halo (cm) | ||||||
---|---|---|---|---|---|---|
Salmonella Typhimurium | Listeria monocytogenes | Escherichia coli | ||||
5–10 K | ≥ 10 K | 5–10 K | ≥ 10 K | 5–10 K | ≥ 10 K | |
CBI1 | 1 | 0.8 | 0 | 0 | 0.8 | 0.8 |
CBI2 | 0 | 0 | 0 | 0 | 0 | 1 |
CBI3 | 0 | 0 | 0 | 0 | 1 | 1 |
CBI4 | 1 | 1 | 0 | 0 | 0 | 0 |
CBI5 | 1 | 1 | 0 | 0 | 1 | 1 |
CBI6 | 3 | 3 | 0 | 0 | 0 | 0 |
CBI7 | 0 | 0 | 0 | 0 | 0 | 0 |
CBI8 | 0 | 0 | 1 | 1 | 0 | 0 |
CBI9 | 1 | 1.5 | 0 | 0 | 1 | 1 |
CBI10 | 0 | 0 | 0 | 0 | 1 | 1 |
CBI11 | 0 | 0 | 0 | 0 | 0 | 0 |
Next Related Type Strain | ||||||
---|---|---|---|---|---|---|
Sample Name | Sample Accession Number | Number of Bases | Accession Code 1 | Accession Code 2 | Accession Code 3 | Highest 16S rRNA Sequence Similarity |
CBI1 | SRX9104367 | 467 | EU257444 (GenBank) | FJ526332 (GenBank) | FJ772081 (GenBank) | Bacillus subtilis (70.83%) |
CBI2 | SRX9104368 | 466 | EU257444 (GenBank) | FJ526332 (GenBank) | FJ772081 (GenBank) | Bacillus subtilis (66.68%) |
CBI3 | SRX9104370 | 440 | HM127153 (GenBank) | HM127159 (GenBank) | HM127158 (GenBank) | Providencia sp. (41.13%) |
CBI4 | SRX9104371 | 440 | AB205631 (GenBank) | JF692663 (GenBank) | KM251257 (GenBank) | Alcaligens sp. (62.40%) |
CBI5 | SRX9104372 | 440 | EF586016 (GenBank) | LK021121 (GenBank) | CCSF01000001 (GenBank) | Pseudomonas sp. 20_BN (78.41%) |
CBI6 | SRX9104373 | 451 | FJ786044 (GenBank) | HQ683838 (GenBank) | CP007626 (NCBI) | Bacillus pseudomycoides (97.73%) |
CBI7 | SRX9104374 | 466 | EU257444 (GenBank) | FJ526332 (GenBank) | FJ772081 (GenBank) | Bacillus subtilis (95.09%) |
CBI8 | SRX9104375 | 451 | JF218251 (GenBank) | Jf218331 (GenBank) | JF218225 (GenBank) | Arthrobacter sp. (95.66%) |
CBI9 | SRX9104376 | 440 | HQ671078 (GenBank) | JX966100 (GenBank) | FJ649673 (GenBank) | Myroides sp. (99.51%) |
CBI10 | SRX9104377 | 440 | FJ672787 (GenBank) | AB673200 (GenBank) | LN565711 (GenBank) | Pseudomonas sp. (54.01%) |
CBI11 | SRX9104369 | 440 | HM127153 (GenBank) | HM127159 (GenBank) | HM127158 (GenBank) | Providencia sp. (48.98%) |
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Tannouri, A.; Rizk, Z.; Al Daccache, M.; Ghanem, C.; Azzi, V.; Haddad, R.; Maroun, R.G.; Hobaika, Z.; Badra, R.; Salameh, D. Characterization of Antagonist Potential of Selected Compost Bacterial Isolates (CBI) against Plant and Human Pathogens. Agronomy 2022, 12, 2977. https://doi.org/10.3390/agronomy12122977
Tannouri A, Rizk Z, Al Daccache M, Ghanem C, Azzi V, Haddad R, Maroun RG, Hobaika Z, Badra R, Salameh D. Characterization of Antagonist Potential of Selected Compost Bacterial Isolates (CBI) against Plant and Human Pathogens. Agronomy. 2022; 12(12):2977. https://doi.org/10.3390/agronomy12122977
Chicago/Turabian StyleTannouri, Abdo, Ziad Rizk, Marina Al Daccache, Chantal Ghanem, Valérie Azzi, Rami Haddad, Richard G. Maroun, Zeina Hobaika, Rebecca Badra, and Dominique Salameh. 2022. "Characterization of Antagonist Potential of Selected Compost Bacterial Isolates (CBI) against Plant and Human Pathogens" Agronomy 12, no. 12: 2977. https://doi.org/10.3390/agronomy12122977