Maternal B Cell-Intrinsic MyD88 Signaling Mediates LPS-Driven Intrauterine Fetal Death
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Husbandry and Model Generation
2.2. Sample Collection and Histology
2.3. Quantitative Histological Measurements
2.4. B Cell Isolation and Stimulation
2.5. Cell Staining and Flow Cytometry
2.6. Cytokine Detection in Sera and Supernatants
2.7. Statistics
3. Results
3.1. MyD88-Deficient B Cells and CD19-Deficient B Cells from Pregnant Mice Have a Defective LPS Response
3.2. Deficient B Cell-Specific MyD88 Signaling Hinders IUFD after LPS Treatment
3.3. Systemic Maternal Imbalance between Pro-Inflammatory and Anti-Inflammatory Processes Alters the Function of Decidual Tissue, Leading to IUFD
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Busse, M.; Plenagl, S.; Campe, N.K.J.; Müller, A.J.; Tedford, K.; Schumacher, A.; Zenclussen, A.C. Maternal B Cell-Intrinsic MyD88 Signaling Mediates LPS-Driven Intrauterine Fetal Death. Cells 2021, 10, 2693. https://doi.org/10.3390/cells10102693
Busse M, Plenagl S, Campe NKJ, Müller AJ, Tedford K, Schumacher A, Zenclussen AC. Maternal B Cell-Intrinsic MyD88 Signaling Mediates LPS-Driven Intrauterine Fetal Death. Cells. 2021; 10(10):2693. https://doi.org/10.3390/cells10102693
Chicago/Turabian StyleBusse, Mandy, Susanne Plenagl, Norina Kim Jutta Campe, Andreas J. Müller, Kerry Tedford, Anne Schumacher, and Ana Claudia Zenclussen. 2021. "Maternal B Cell-Intrinsic MyD88 Signaling Mediates LPS-Driven Intrauterine Fetal Death" Cells 10, no. 10: 2693. https://doi.org/10.3390/cells10102693