The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Ethics
2.2. Calculation of the Expression Levels of LTR Elements and Genes
2.3. Oocyte Collection and In Vitro Fertilization
2.4. Lentivirus Production and Purification
2.5. Isolation of Green Fluorescent Protein (GFP) Positive Single Cell and scRNA-Seq
2.6. Single-Cell Cluster Analysis
2.7. TF Binding Site Predictions and Dual Luciferase Expression Assays
2.8. Immunofluorescence
3. Results
3.1. Temporal Expression of LTR Elements during Monkey Embryo Development
3.2. MacERV6-LTR1a Knockdown in Cynomolgus Monkey Embryos
3.3. Transcriptomic Changes after MacERV6-LTR1a Knockdown
3.4. The Interaction between MacERV6-LTR1a and Pluripotency Factors In Vitro
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
TF | Transcription factor |
TrB | Trophectoderm |
TE | Transposable Element |
ICM | Inner cell mass |
EPI | Epiblast |
preEEPI | Pre-implantation early epiblast |
preETE | Pre-implantation early trophectoderm |
Pre-EPI | Pre-implantation epiblast |
postE-EPI | Post-implantation early epiblast |
postL-EPI | Post-implantation late epiblast |
PrE | Primitive endoderm |
ERV | Endogenous retroviruse |
LTR | Long terminal repeat |
hESC | Human embryonic stem cell |
KRAB-ZFP | KRAB-containing zinc finger protein |
rhFSH | Recombinant human follitropin alpha |
rhCG | Recombinant human chorionic gonadotropin alpha |
TALP | Tyrode’s albumin lactate pyruvate |
BSA | Bovine serum albumin |
MOR | Morula |
DEGs | Differentially expressed genes |
Gast1 | Gastrulating cells 1 |
Gast2a | Gastrulating cells 2a |
Gast2b | Gastrulating cells 2b |
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Duan, K.; Si, C.-Y.; Zhao, S.-M.; Ai, Z.-Y.; Niu, B.-H.; Yin, Y.; Xiang, L.-F.; Ding, H.; Zheng, Y. The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey. Cells 2021, 10, 2710. https://doi.org/10.3390/cells10102710
Duan K, Si C-Y, Zhao S-M, Ai Z-Y, Niu B-H, Yin Y, Xiang L-F, Ding H, Zheng Y. The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey. Cells. 2021; 10(10):2710. https://doi.org/10.3390/cells10102710
Chicago/Turabian StyleDuan, Kui, Chen-Yang Si, Shu-Mei Zhao, Zong-Yong Ai, Bao-Hua Niu, Yu Yin, Li-Feng Xiang, Hao Ding, and Yun Zheng. 2021. "The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey" Cells 10, no. 10: 2710. https://doi.org/10.3390/cells10102710