Effect of shRNA Mediated Silencing of YB-1 Protein on the Expression of Matrix Collagenases in Malignant Melanoma Cell In Vitro
Abstract
:1. Introduction
2. Results
2.1. YB-1 Silencing via shRNA Mediated RNA Interference in A375 Malignant Melanoma Cell Line
2.2. Antiproliferative Effect of YB-1 Silencing in A375 Cell Line
2.3. Effect of YB-1 Silencing on Expression of Matrix Collagenases in A375 Cell Line
2.4. Effect of YB-1 Silencing on A375 Cell Line Migration Assay In Vitro
3. Discussion
3.1. YB-1 Protein and A375 Cancer Cell Proliferation
3.2. YB-1 Protein and the Expression of Collagenases MMPs in A375 Cancer Cells
3.3. YB-1 Protein and A375 Cancer Cell Migration
4. Materials and Methods
4.1. Establishing the Stable YB-1 Silenced Malignant Melanoma Cell Line
4.2. Real-Time PCR
4.3. Protein Extraction and Western Blot Analysis
4.4. MTT Assay and Cell Counting
4.5. Immuno-Fluorescence Staining
4.6. Hoechst Nuclear Staining
4.7. Flow Cytometry for Cell Cycle Interference
4.8. Wound Healing Assay
4.9. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Ibrahim, W.N.; Doolaanea, A.A.; Bin Abdull Rasad, M.S.B. Effect of shRNA Mediated Silencing of YB-1 Protein on the Expression of Matrix Collagenases in Malignant Melanoma Cell In Vitro. Cells 2018, 7, 7. https://doi.org/10.3390/cells7010007
Ibrahim WN, Doolaanea AA, Bin Abdull Rasad MSB. Effect of shRNA Mediated Silencing of YB-1 Protein on the Expression of Matrix Collagenases in Malignant Melanoma Cell In Vitro. Cells. 2018; 7(1):7. https://doi.org/10.3390/cells7010007
Chicago/Turabian StyleIbrahim, Wisam Nabeel, Abd Almonem Doolaanea, and Mohammad Syaiful Bahari Bin Abdull Rasad. 2018. "Effect of shRNA Mediated Silencing of YB-1 Protein on the Expression of Matrix Collagenases in Malignant Melanoma Cell In Vitro" Cells 7, no. 1: 7. https://doi.org/10.3390/cells7010007