Role of Wnt Signaling During In-Vitro Bovine Blastocyst Development and Maturation in Synergism with PPARδ Signaling
Abstract
:1. Introduction
2. Materials and Methods
2.1. Drug Treatment for Wnt Stimulation
2.2. Drug Treatment for PPARδ Inhibition
2.3. Aspiration of Oocyte and In-Vitro Maturation
2.4. In-Vitro Fertilization and In-Vitro Culture of Embryos
2.5. RNA Extraction and Quantitative Real Time PCR (q-RT-PCR) Analysis
2.6. Immunofluorescence Analysis
2.7. Quantification of Lipid Content by Fluorescent Probe Nile Red
2.8. Invasion Assay
2.9. Statistical Analysis
3. Results
3.1. Augmentation of Canonical Wnt Activity via 6-Bio Treatment Enhances Bovine BL Development and Hatching
3.2. Effect of 6-Bio Treatment on Proliferation and Lineage Specification during BL Development
3.3. Exogenous Stimulation of Canonical Wnt Activity Induces PPARδ Signaling during Bovine Embryonic Development
3.4. Wnt Regulate BL Development by Sustaining Proliferative Signaling via Modulating PPARδ Expression
3.5. Wnt Promote BL Development and Hatching via Regulating FAO Metabolism through PPARδ Signaling
3.6. Canonical Wnt Activation in Co-ordination with PPARδ Reduces Lipid Content and Enhances BL Implantation Potential
3.7. Exogenous Stimulation of Canonical Wnt Activity Promote Co-localization of PPARδ with β-Catenin during Bovine Embryonic Development
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Groups (nM) | Oocytes, n | Speculated Zygotes, n | Cleaved Embryos, % | Total Blastocyst, % | Hatched Blastocyst, % |
---|---|---|---|---|---|
Control | 340 | 315 | 245 (77.8 ± 0.5) b | 93 (29.6 ± 0.5) b | 41 (44.9 ± 1.8) b |
6-Bio (200) | 295 | 277 | 217 (78.3 ± 0.6) b | 94 (33.9 ± 0.9) c | 53 (55.9 ± 3.4) c |
6-Bio (300) | 313 | 290 | 227 (78.3 ± 0.3) b | 99 (34.1 ± 1.1) c | 56 (55.9 ± 3.4) c |
6-Bio (400) | 346 | 321 | 254 (78.9 ± 0.4) b | 121 (37.8 ± 0.5) d | 78 (64.3 ± 1.2) c |
6-Bio (500) | 165 | 151 | 83 (55.0 ± 1.7) a | 31 (20.8 ± 1.3) a | 7 (22.5 ± 3.2) a |
Groups | Oocytes, n | Speculated Zygotes, n | Cleaved Embryos, % | Total Blastocyst, % | Hatched Blastocyst, % |
---|---|---|---|---|---|
Control | 502 | 481 | 377 (78.5 ± 0.2) c | 140 (29.2 ± 0.5 ) c | 63 (45.0 ± 0.9) c |
6-Bio (400 nM) | 521 | 496 | 387 (78.2 ± 0.3) c | 188 (37.8 ± 1.3) d | 115 (61.1 ± 1.6) d |
Gsk3787 (15 µM) | 453 | 430 | 323 (75.0 ± 0.4) a | 69 (16.1 ± 0.6) a | 5 (7.2 ± 2.5) a |
6-Bio + Gsk3787 | 521 | 494 | 378 (76.7 ± 0.4) b | 120 (24.3 ± 0.4 ) b | 43 (35.5 ± 1.2) b |
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Sidrat, T.; Khan, A.A.; Idrees, M.; Joo, M.-D.; Xu, L.; Lee, K.-L.; Kong, I.-K. Role of Wnt Signaling During In-Vitro Bovine Blastocyst Development and Maturation in Synergism with PPARδ Signaling. Cells 2020, 9, 923. https://doi.org/10.3390/cells9040923
Sidrat T, Khan AA, Idrees M, Joo M-D, Xu L, Lee K-L, Kong I-K. Role of Wnt Signaling During In-Vitro Bovine Blastocyst Development and Maturation in Synergism with PPARδ Signaling. Cells. 2020; 9(4):923. https://doi.org/10.3390/cells9040923
Chicago/Turabian StyleSidrat, Tabinda, Abdul Aziz Khan, Muhammad Idrees, Myeong-Don Joo, Lianguang Xu, Kyeong-Lim Lee, and Il-Keun Kong. 2020. "Role of Wnt Signaling During In-Vitro Bovine Blastocyst Development and Maturation in Synergism with PPARδ Signaling" Cells 9, no. 4: 923. https://doi.org/10.3390/cells9040923