Granulocyte Colony Stimulating Factor (GCSF) Can Attenuate Neuropathic Pain by Suppressing Monocyte Chemoattractant Protein-1 (MCP-1) Expression, through Upregulating the Early MicroRNA-122 Expression in the Dorsal Root Ganglia
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Surgical Procedure and GCSF Treatment Protocol
2.3. Behavioral Tests for Mechanical Allodynia
2.4. MicroRNA Isolation and Purification
2.5. nCounter Data Analysis
2.6. Enzyme-Linked Immunosorbent Assay (ELISA)
2.7. Immunohistochemistry (IHC) Studies
2.8. Statistical Analysis
3. Results
3.1. Single Early Systemic GCSF Treatment Alleviated Mechanical Allodynia in CCI Rats
3.2. GCSF Upregulated MicroRNA-122 Expression in the DRGs of CCI Rats on the 1st Day after Nerve Injury
3.3. GCSF Decreased MCP-1 Expression in the DRGs of CCI Rats on the 7th Day after Nerve Injury
4. Discussion
4.1. GCSF Upregulated MicroRNA-122 Expression in the DRGs of CCI Rats at the Early Phase after Nerve Injury
4.2. MicroRNA-122 Can Suppress the Pro-Inflammatory Cytokines and MCP-1 Expressions in an Ex Vivo Study
4.3. MCP-1 in the DRGs and SDHs Plays an Important Role to Promote Neuropathic Pain Formation
4.4. GCSF Suppressed MCP-1 Expression in the DRGs to Attenuate Neuropathic Pain
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Liao, M.-F.; Hsu, J.-L.; Lu, K.-T.; Chao, P.-K.; Cheng, M.-Y.; Hsu, H.-C.; Lo, A.-L.; Lee, Y.-L.; Hung, Y.-H.; Lyu, R.-K.; et al. Granulocyte Colony Stimulating Factor (GCSF) Can Attenuate Neuropathic Pain by Suppressing Monocyte Chemoattractant Protein-1 (MCP-1) Expression, through Upregulating the Early MicroRNA-122 Expression in the Dorsal Root Ganglia. Cells 2020, 9, 1669. https://doi.org/10.3390/cells9071669
Liao M-F, Hsu J-L, Lu K-T, Chao P-K, Cheng M-Y, Hsu H-C, Lo A-L, Lee Y-L, Hung Y-H, Lyu R-K, et al. Granulocyte Colony Stimulating Factor (GCSF) Can Attenuate Neuropathic Pain by Suppressing Monocyte Chemoattractant Protein-1 (MCP-1) Expression, through Upregulating the Early MicroRNA-122 Expression in the Dorsal Root Ganglia. Cells. 2020; 9(7):1669. https://doi.org/10.3390/cells9071669
Chicago/Turabian StyleLiao, Ming-Feng, Jung-Lung Hsu, Kwok-Tung Lu, Po-Kuan Chao, Mei-Yun Cheng, Hui-Ching Hsu, Ai-Lun Lo, Yun-Lin Lee, Yu-Hui Hung, Rong-Kuo Lyu, and et al. 2020. "Granulocyte Colony Stimulating Factor (GCSF) Can Attenuate Neuropathic Pain by Suppressing Monocyte Chemoattractant Protein-1 (MCP-1) Expression, through Upregulating the Early MicroRNA-122 Expression in the Dorsal Root Ganglia" Cells 9, no. 7: 1669. https://doi.org/10.3390/cells9071669