Formation of Aberrant Myotubes by Myoblasts Lacking Myosin VI Is Associated with Alterations in the Cytoskeleton Organization, Myoblast Adhesion and Fusion
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Myoblast Isolation
2.3. Microscopy and Imaging
2.4. Immunoblotting
2.5. Antibodies and Fluorescent Markers
2.6. Cell Cycle Analysis
2.7. Statistical Analysis
3. Results
3.1. Aberrant Myotube Formation by Myoblasts Lacking MVI
3.2. Cell Adhesion Is Impaired in Myoblasts Lacking MVI
3.3. Expression Pattern of Myomaker and Myomerger Is Altered During Differentiation of Myoblasts Lacking MVI
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Lehka, L.; Topolewska, M.; Wojton, D.; Karatsai, O.; Alvarez-Suarez, P.; Pomorski, P.; Rędowicz, M.J. Formation of Aberrant Myotubes by Myoblasts Lacking Myosin VI Is Associated with Alterations in the Cytoskeleton Organization, Myoblast Adhesion and Fusion. Cells 2020, 9, 1673. https://doi.org/10.3390/cells9071673
Lehka L, Topolewska M, Wojton D, Karatsai O, Alvarez-Suarez P, Pomorski P, Rędowicz MJ. Formation of Aberrant Myotubes by Myoblasts Lacking Myosin VI Is Associated with Alterations in the Cytoskeleton Organization, Myoblast Adhesion and Fusion. Cells. 2020; 9(7):1673. https://doi.org/10.3390/cells9071673
Chicago/Turabian StyleLehka, Lilya, Małgorzata Topolewska, Dominika Wojton, Olena Karatsai, Paloma Alvarez-Suarez, Paweł Pomorski, and Maria Jolanta Rędowicz. 2020. "Formation of Aberrant Myotubes by Myoblasts Lacking Myosin VI Is Associated with Alterations in the Cytoskeleton Organization, Myoblast Adhesion and Fusion" Cells 9, no. 7: 1673. https://doi.org/10.3390/cells9071673