DNA Methylation of Postnatal Liver Development in Pigs
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Animal Experiment and Sample Preparation
2.3. RNA-Seq
2.4. Library Preparation and Bisulfite Sequencing
2.5. Sequencing Read Quality Control and Alignment
2.6. Detection and Analysis of Methylation Sites
2.7. Correlation Analysis and PCA
2.8. DMR Analysis
2.9. Functional Enrichment Analysis for Genes
3. Results
3.1. Data Quality of BS-Seq Data
3.2. Methylation Distribution Characteristics
3.3. Characterization of Porcine Liver Transcriptome
3.4. Methylation Profile of Porcine Liver
3.4.1. Distribution Characteristics of DMRs
3.4.2. The DMRs of the Promoter
3.4.3. Genes with Differential Expression in the Promoter DMR
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Wang, Y.; Jin, H.; Tong, X.; Yu, H.; Li, X.; Zeng, B. DNA Methylation of Postnatal Liver Development in Pigs. Genes 2024, 15, 1067. https://doi.org/10.3390/genes15081067
Wang Y, Jin H, Tong X, Yu H, Li X, Zeng B. DNA Methylation of Postnatal Liver Development in Pigs. Genes. 2024; 15(8):1067. https://doi.org/10.3390/genes15081067
Chicago/Turabian StyleWang, Yuhao, Hongling Jin, Xingyan Tong, Huan Yu, Xuewei Li, and Bo Zeng. 2024. "DNA Methylation of Postnatal Liver Development in Pigs" Genes 15, no. 8: 1067. https://doi.org/10.3390/genes15081067