Wnt Effector TCF4 Is Dispensable for Wnt Signaling in Human Cancer Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Mice
2.2. Cell and Organoid Culture; 4-Hydroxytamoxifen Treatment
2.3. Cell Transfection, Small Interfering RNA, and Immunoblotting
2.4. RNA Isolation and Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR)
2.5. Fluorescent Microscopy
2.6. Fluorescence-Activated Cell Sorting
2.7. Immunohistochemistry and β-Galactosidase Staining
2.8. Statistical Analysis
3. Results and Discussion
3.1. Analysis of Tcf4 Expression in the Mouse Small Intestine and Colon
3.2. Intestinal Epithelium-Specific Tcf4 Inactivation Had No Effect on the Embryonic Gut
3.3. Absence of Tcf4 Compromised Cell Proliferation of the Adult Small Intestinal and Colon Epithelia
3.4. Tcf4-Deficient Intestinal Stem Cells s Do Not Contribute to Intestinal Homeostasis
3.5. Tcf4 Loss Affects the Size of Apc-Deficient Small Intestinal Tumors
3.6. Tcf4-Deficient Organoids Displayed Impaired Growth
3.7. Redundancy of LEF/TCF Family Members in Human APC-Deficient Cells
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Hrckulak, D.; Janeckova, L.; Lanikova, L.; Kriz, V.; Horazna, M.; Babosova, O.; Vojtechova, M.; Galuskova, K.; Sloncova, E.; Korinek, V. Wnt Effector TCF4 Is Dispensable for Wnt Signaling in Human Cancer Cells. Genes 2018, 9, 439. https://doi.org/10.3390/genes9090439
Hrckulak D, Janeckova L, Lanikova L, Kriz V, Horazna M, Babosova O, Vojtechova M, Galuskova K, Sloncova E, Korinek V. Wnt Effector TCF4 Is Dispensable for Wnt Signaling in Human Cancer Cells. Genes. 2018; 9(9):439. https://doi.org/10.3390/genes9090439
Chicago/Turabian StyleHrckulak, Dusan, Lucie Janeckova, Lucie Lanikova, Vitezslav Kriz, Monika Horazna, Olga Babosova, Martina Vojtechova, Katerina Galuskova, Eva Sloncova, and Vladimir Korinek. 2018. "Wnt Effector TCF4 Is Dispensable for Wnt Signaling in Human Cancer Cells" Genes 9, no. 9: 439. https://doi.org/10.3390/genes9090439