Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for Accurate and Prompt Coronavirus Disease 2019 (COVID-19) Diagnosis Using the Rational Selection of Serological Biomarkers
Abstract
:1. Introduction
2. Study Design
2.1. Study Population
2.2. Proteins
2.3. Selection of the Most Immunoreactive Protein Antigens
2.4. Selection of the Most Immunoreactive Combination of Protein Antigens
2.5. IgA and IgM Assessment
2.6. Determining Inter-Assay Variability and Repeatability
2.7. Sera Heat Treatment for Viral Inactivation
2.8. Inhibition of IgG Antibodies against Three Antigens
2.9. Comparing the “In-House” SARS-CoV-2 ELISA to Commercially Available Assays
2.10. Implementation of the Developed ELISA in PCR-Positive Children
2.11. Statistical Analysis
3. Results
3.1. ELISA Development
3.2. Optimization of SARS-CoV-2 Protein Coating Concentration and Serum Dilution
3.3. IgA and IgM Antibody Detection
3.4. Determining Inter-Assay and Intra-Assay Variability and Repeatability
3.5. Inhibition of IgG Antibody Binding
3.6. Comparing the In-House SARS-CoV-2 ELISA to Commercially Available Assays
3.7. Evaluation of the “In-House” ELISA in Children
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Lagousi, T.; Routsias, J.; Spoulou, V. Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for Accurate and Prompt Coronavirus Disease 2019 (COVID-19) Diagnosis Using the Rational Selection of Serological Biomarkers. Diagnostics 2021, 11, 1970. https://doi.org/10.3390/diagnostics11111970
Lagousi T, Routsias J, Spoulou V. Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for Accurate and Prompt Coronavirus Disease 2019 (COVID-19) Diagnosis Using the Rational Selection of Serological Biomarkers. Diagnostics. 2021; 11(11):1970. https://doi.org/10.3390/diagnostics11111970
Chicago/Turabian StyleLagousi, Theano, John Routsias, and Vana Spoulou. 2021. "Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for Accurate and Prompt Coronavirus Disease 2019 (COVID-19) Diagnosis Using the Rational Selection of Serological Biomarkers" Diagnostics 11, no. 11: 1970. https://doi.org/10.3390/diagnostics11111970