A Novel Genetic Sexing Strain of Anastrepha ludens for Cost-Effective Sterile Insect Technique Applications: Improved Genetic Stability and Rearing Efficiency
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
3. Results
3.1. Induction of New Translocations and Selection of New Genetic Sexing Strain (GSS)
3.2. Fecundity and Fertility
3.3. Genetic Stability and Productivity of the Selected GSS GUA10
3.4. Quality Control Analysis under Laboratory Conditions
3.5. Irradiation doses Response Curve for GUA10
3.6. Field Cage Tests
3.7. Cytogenetic Analysis of GUA10 Translocation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Family | n | % Recombinants (♀ in Brown Pupae) | % Recombinants (♂ in Black Pupae) | Egg to Pupae Conversion Rate (E:P) |
---|---|---|---|---|
197 | 6 | 25.03 a ± 1.397 | 22.19 a ± 1.677 | 0.36 c ± 0.005 × |
109 | 17 | 0.28 c ± 0.153 | 0.20 c ± 0.110 | 0.31 c ± 0.019 × |
48 | 6 | 5.30 b ± 2.672 | 9.97 b ± 4.538 | 0.30 c ± 0.028 × |
45 | 14 | 0.27 c ± 0.220 | 0.00 c ± 0.000 | 0.34 c ± 0.016 × |
9 | 17 | 2.80 b ± 1.569 | 0.97 c ± 0.533 | 0.29 c ± 0.019 × |
87 | 17 | 0.00 c ± 0.000 | 0.00 c ± 0.000 | 0.34 c ± 0.012 ×× |
125 | 17 | 0.00 c ± 0.000 | 0.00 c ± 0.000 | 0.33 c ± 0.019 ×× |
116 | 17 | 0.00 c ± 0.000 | 0.00 c ± 0.000 | 0.32 c ± 0.013 ×× |
154 | 17 | 0.00 c ± 0.000 | 0.00 c ± 0.000 | 0.32 c ± 0.014 ×× |
103 | 17 | 0.00 c ± 0.000 | 0.00 c ± 0.000 | 0.32 c ± 0.025 ×× |
66 | 17 | 0.00 c ± 0.000 | 0.00 c ± 0.000 | 0.31 c ± 0.017 ×× |
10 | 17 | 0.00 c ± 0.000 | 0.00 c ± 0.000 | 0.41 b ± 0.016 ××× |
TAP7 (Control) | 17 | 3.02 b ± 0.725 | 0.56 c ± 0.202 | 0.32 c ± 0.015 |
Wild (Reference) | 13 | – | – | 0.74 a ± 0.029 |
Cross | Low Density (without Stress) | High Density (with Stress) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Male | × | Female | n | Eggs/♀/Day | n | % Egg Hatch | n | Eggs/♀/Day | n | % Egg Hatch |
GUA10 | × | GUA10 | 90 | 49.50 a± 1.33 | 105 | 86.92 a± 0.71 | 14 | 29.14 a± 2.30 | 10 | 85.54 a± 1.33 |
TAP7 | × | TAP7 | 82 | 25.57 b± 1.39 | 96 | 74.68 b± 0.74 | 12 | 22.75 a± 2.49 | 9 | 70.67 b± 1.40 |
Scale | Strain | Larval Recovery | Egg: Pupae Conversion Efficiency | Millions of Pupae/ton of Larval Diet |
---|---|---|---|---|
Medium | GUA10 | 0.18 a ± 0.003 | 0.26 a ± 0.01 | 4.17 a ± 0.102 |
TAP7 | 0.13 b ± 0.007 | 0.17 b ± 0.008 | 2.81 b ± 0.090 | |
Large | GUA10 | 0.14 a ± 0.003 | 0.28 a ± 0.005 | 3.60 a ± 0.081 |
TAP7 | 0.11 b ± 0.005 | 0.21 b ± 0.009 | 2.78 b ± 0.117 |
Males (Brown Pupae) | Females (Black Pupae) | Recombinants | |||
---|---|---|---|---|---|
Strain | Emergence | Flyers | Emergence | Flyers | (♀ in Brown Pupae) |
GUA10 | 93.36 a ± 0.652 | 89.62 a± 0.924 | 89.54 a ± 0.618 | 81.65 a ± 1.033 | 1.25 a ± 0.199 |
TAP7 | 78.67 b ± 0.681 | 78.76 b ± 0.364 | 81.59 b ± 0.571 | 77.37 b ± 0.669 | 12.65 b ± 1.740 |
Cross | ||||||||
---|---|---|---|---|---|---|---|---|
(♂GUA10 × ♂TAP7 × ♂w) × ♀w | (♂GUA10 × ♂w) × ♀w | (♂TAP7 × ♂w) × ♀w | ||||||
Male | n | % Matings ± SE | RMPI | n | % Matings ± SE | RSI | n | % Matings ± SE |
GUA10 | 20 | 19.10 b ± 2.32 | 0.40 a ± 0.03 | 22 | 30.73 a ± 3.29 | 0.68 a ± 0.03 | ||
TAP7 | 20 | 16.30 b ± 2.27 | 0.32 b ± 0.02 | 0.54 b ± 0.02 | 22 | 22.73 b ± 2.56 | ||
W | 20 | 14.70 b ± 2.26 | 22 | 16.09 b ± 2.82 | 22 | 20.55 b ± 3.07 | ||
Control | 11 | 51.27 a ± 8.55 | 11 | 41.45 a ± 7.37 | 11 | 41.45 a ± 7.37 |
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Ramírez-Santos, E.; Rendon, P.; Gouvi, G.; Zacharopoulou, A.; Bourtzis, K.; Cáceres, C.; Bloem, K. A Novel Genetic Sexing Strain of Anastrepha ludens for Cost-Effective Sterile Insect Technique Applications: Improved Genetic Stability and Rearing Efficiency. Insects 2021, 12, 499. https://doi.org/10.3390/insects12060499
Ramírez-Santos E, Rendon P, Gouvi G, Zacharopoulou A, Bourtzis K, Cáceres C, Bloem K. A Novel Genetic Sexing Strain of Anastrepha ludens for Cost-Effective Sterile Insect Technique Applications: Improved Genetic Stability and Rearing Efficiency. Insects. 2021; 12(6):499. https://doi.org/10.3390/insects12060499
Chicago/Turabian StyleRamírez-Santos, Edwin, Pedro Rendon, Georgia Gouvi, Antigone Zacharopoulou, Kostas Bourtzis, Carlos Cáceres, and Kenneth Bloem. 2021. "A Novel Genetic Sexing Strain of Anastrepha ludens for Cost-Effective Sterile Insect Technique Applications: Improved Genetic Stability and Rearing Efficiency" Insects 12, no. 6: 499. https://doi.org/10.3390/insects12060499