The Effects of Aspirin Intervention on Inflammation-Associated Lingual Bacteria: A Pilot Study from a Randomized Clinical Trial

Onyeaghala, Guillaume C.; Sharma, Shweta; Oyenuga, Mosunmoluwa; Staley, Christopher M.; Milne, Ginger L.; Demmer, Ryan T.; Shaukat, Aasma; Thyagarajan, Bharat; Straka, Robert J.; Church, Timothy R.; Prizment, Anna E.

doi:10.3390/microorganisms12081609

Open AccessArticle

The Effects of Aspirin Intervention on Inflammation-Associated Lingual Bacteria: A Pilot Study from a Randomized Clinical Trial

by

Guillaume C. Onyeaghala

¹

,

Shweta Sharma

²

,

Mosunmoluwa Oyenuga

³,

Christopher M. Staley

^4,5

,

Ginger L. Milne

⁶,

Ryan T. Demmer

⁷,

Aasma Shaukat

⁸,

Bharat Thyagarajan

^2,9

,

Robert J. Straka

¹⁰

,

Timothy R. Church

^5,11

and

Anna E. Prizment

^2,9,*

¹

Division of Nephrology, Hennepin Healthcare, University of Minnesota, Minneapolis, MN 55415, USA

²

Division of Epidemiology and Community Health, University of Minnesota, Minneapolis, MN 55455, USA

³

Department of Internal Medicine, SSM Health St. Mary’s Hospital—St. Louis, St. Louis, MO 63117, USA

⁴

Department of Surgery, Medical School, University of Minnesota, Minneapolis, MN 55455, USA

⁵

Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA

⁶

Department of Medicine, Vanderbilt School of Medicine, Nashville, TN 37232, USA

⁷

Mayo Clinic College of Medicine & Sciences, Rochester, MN 55905, USA

⁸

Department of Population Health, New York University Grossman School of Medicine, New York University, New York, NY 10016, USA

⁹

Department of Laboratory Medicine & Pathology, Medical School, University of Minnesota, Minneapolis, MN 55455, USA

¹⁰

Department of Experimental and Clinical Pharmacology, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA

¹¹

Division of Environmental Health Sciences, School of Public Health, University of Minnesota, Saint Paul, MN 55108, USA

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^*

Author to whom correspondence should be addressed.

Microorganisms 2024, 12(8), 1609; https://doi.org/10.3390/microorganisms12081609

Submission received: 24 July 2024 / Revised: 2 August 2024 / Accepted: 4 August 2024 / Published: 7 August 2024

(This article belongs to the Special Issue Gut Microbiome in Homeostasis and Disease, 2nd Edition)

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Abstract

Several bacterial taxa enriched in inflammatory bowel diseases and colorectal cancer (CRC) are found in the oral cavity. We conducted a pilot study nested within a six-week aspirin intervention in a randomized placebo-controlled trial to test their response to aspirin intervention. Fifty healthy subjects, 50–75 years old, were randomized to receive 325 mg aspirin (n = 30) or placebo (n = 20) orally once daily for six weeks. Oral tongue swabs were collected at baseline and week six. We estimated the association between aspirin use and the temporal changes in the relative abundance of pre-specified genus level taxa from pre- to post-treatment. The temporal change in relative abundance differed for eight genus level taxa between the aspirin and placebo groups. In the aspirin group, there were significant increases in the relative abundances of Neisseria, Streptococcus, Actinomyces, and Rothia and significant decreases in Prevotella, Veillonella, Fusobacterium, and Porphyromonas relative to placebo. The log ratio of Neisseria to Fusobacterium declined more in the aspirin group than placebo, signaling a potential marker associated with aspirin intervention. These preliminary findings should be validated using metagenomic sequencing and may guide future studies on the role of aspirin on taxa in various oral ecological niches.

Keywords: oral microbiome; periodontitis; colorectal cancer; aspirin

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MDPI and ACS Style

Onyeaghala, G.C.; Sharma, S.; Oyenuga, M.; Staley, C.M.; Milne, G.L.; Demmer, R.T.; Shaukat, A.; Thyagarajan, B.; Straka, R.J.; Church, T.R.; et al. The Effects of Aspirin Intervention on Inflammation-Associated Lingual Bacteria: A Pilot Study from a Randomized Clinical Trial. Microorganisms 2024, 12, 1609. https://doi.org/10.3390/microorganisms12081609

AMA Style

Onyeaghala GC, Sharma S, Oyenuga M, Staley CM, Milne GL, Demmer RT, Shaukat A, Thyagarajan B, Straka RJ, Church TR, et al. The Effects of Aspirin Intervention on Inflammation-Associated Lingual Bacteria: A Pilot Study from a Randomized Clinical Trial. Microorganisms. 2024; 12(8):1609. https://doi.org/10.3390/microorganisms12081609

Chicago/Turabian Style

Onyeaghala, Guillaume C., Shweta Sharma, Mosunmoluwa Oyenuga, Christopher M. Staley, Ginger L. Milne, Ryan T. Demmer, Aasma Shaukat, Bharat Thyagarajan, Robert J. Straka, Timothy R. Church, and et al. 2024. "The Effects of Aspirin Intervention on Inflammation-Associated Lingual Bacteria: A Pilot Study from a Randomized Clinical Trial" Microorganisms 12, no. 8: 1609. https://doi.org/10.3390/microorganisms12081609

APA Style

Onyeaghala, G. C., Sharma, S., Oyenuga, M., Staley, C. M., Milne, G. L., Demmer, R. T., Shaukat, A., Thyagarajan, B., Straka, R. J., Church, T. R., & Prizment, A. E. (2024). The Effects of Aspirin Intervention on Inflammation-Associated Lingual Bacteria: A Pilot Study from a Randomized Clinical Trial. Microorganisms, 12(8), 1609. https://doi.org/10.3390/microorganisms12081609

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The Effects of Aspirin Intervention on Inflammation-Associated Lingual Bacteria: A Pilot Study from a Randomized Clinical Trial

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