Impact of Graphene Layers on Genetic Expression and Regulation within Sulfate-Reducing Biofilms
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Inoculation and Cultivation
2.2. Graphene Growth
2.3. Construction of the O. alaskensis G20 (pBMC6 pC3::gfp) Strain
2.4. Sample Preparation for Confocal Microscopy
2.5. Crystal Violet Staining for Bacterial Biofilm Grown on Metal Surfaces
2.6. Colony Counting Method
2.7. RNA Extraction
2.8. cDNA Synthesis and qPCR
2.9. Statistical Analysis
3. Results and Discussion
3.1. Effects of Graphene Layer on gfp-O. alaskensis G20 Adhesion and Biofilm Formation
3.2. O. alaskensis G20 Culturability and Biofilm Quantification on Surfaces
3.3. Expression Levels of Biofilm Genes in CuF, Cu-GrI, and Cu-GrII Surfaces
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gopalakrishnan, V.; Saxena, P.; Thakur, P.; Lipatov, A.; Sani, R.K. Impact of Graphene Layers on Genetic Expression and Regulation within Sulfate-Reducing Biofilms. Microorganisms 2024, 12, 1759. https://doi.org/10.3390/microorganisms12091759
Gopalakrishnan V, Saxena P, Thakur P, Lipatov A, Sani RK. Impact of Graphene Layers on Genetic Expression and Regulation within Sulfate-Reducing Biofilms. Microorganisms. 2024; 12(9):1759. https://doi.org/10.3390/microorganisms12091759
Chicago/Turabian StyleGopalakrishnan, Vinoj, Priya Saxena, Payal Thakur, Alexey Lipatov, and Rajesh K. Sani. 2024. "Impact of Graphene Layers on Genetic Expression and Regulation within Sulfate-Reducing Biofilms" Microorganisms 12, no. 9: 1759. https://doi.org/10.3390/microorganisms12091759