AfSwi6 Regulates the Stress Response, Chlamydospore Production, and Pathogenicity in the Nematode-Trapping Fungus Arthrobotrys flagrans
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Culture
2.2. Sequence and Phylogenetic Analysis
2.3. Plasmid Construction and Protoplast Transformation
2.4. Hyphae Growth and Analysis of Resistance
2.5. Hyphae Morphology, Lipid Droplets, and Glycogen Staining
2.6. Trap Formation and Assays of Pathogenicity
2.7. Transcriptome Sequencing
2.8. Real-Time Fluorescent Quantitative PCR Assay
2.9. Statistical Analyses
3. Results
3.1. Analysis of the Phylogenetic Tree and Protein Conserved Domains and the Deletion of AfSwi6
3.2. AfSwi6 Affects Fungal Growth and Resistance to Stress
3.3. AfSwi6 Is Vital for Chlamydospore Formation and the Accumulation of Glycogen and Lipid Droplets
3.4. AfSwi6 Plays a Key Role in Trap Formation and Pathogenicity in A. flagrans
3.5. Transcriptomic Analysis of the WT and ΔAfSwi6
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Linghu, S.-X.; Zhang, Y.; Zuo, J.-F.; Mo, M.-H.; Li, G.-H. AfSwi6 Regulates the Stress Response, Chlamydospore Production, and Pathogenicity in the Nematode-Trapping Fungus Arthrobotrys flagrans. Microorganisms 2024, 12, 1765. https://doi.org/10.3390/microorganisms12091765
Linghu S-X, Zhang Y, Zuo J-F, Mo M-H, Li G-H. AfSwi6 Regulates the Stress Response, Chlamydospore Production, and Pathogenicity in the Nematode-Trapping Fungus Arthrobotrys flagrans. Microorganisms. 2024; 12(9):1765. https://doi.org/10.3390/microorganisms12091765
Chicago/Turabian StyleLinghu, Shao-Xiang, Yu Zhang, Jia-Fang Zuo, Ming-He Mo, and Guo-Hong Li. 2024. "AfSwi6 Regulates the Stress Response, Chlamydospore Production, and Pathogenicity in the Nematode-Trapping Fungus Arthrobotrys flagrans" Microorganisms 12, no. 9: 1765. https://doi.org/10.3390/microorganisms12091765