New Insights into Microbial Diversity of the Traditional Packed Table Olives Aloreña de Málaga through Metataxonomic Analysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Packed Aloreña de Málaga
2.2. Physicochemical and Microbiological Analyses
2.3. DNA Extraction from Table Olive Samples and Library Preparation
2.4. Bioinformatic Analysis
3. Results
3.1. Physicochemical Analysis
3.2. Microbiological Analysis
3.3. Metataxonomic Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bacteria Population | |||||
Time | Observed | FaithPD | Shannon | Simpson | Clonality |
T0 (n = 4) | 42.25 ± 1.71 | 1.32 ± 0.09 | 2.09 ± 0.12 | 0.79 ± 0.04 | 0.95 ± 0.00 |
T1 (n = 4) | 40.67 ± 2.08 | 1.19 ± 0.25 | 2.06 ± 0.06 | 0.78 ± 0.02 | 0.95 ± 0.00 |
T2 (n = 3) | 45.00 ± 4.83 | 1.36 ± 0.08 | 2.20 ± 0.04 | 0.81 ± 0.01 | 0.95 ± 0.00 |
T3 (n = 4) | 47.50 ± 15.78 | 0.98 ± 0.11 | 2.00 ± 0.46 | 0.70 ± 0.15 | 0.96 ± 0.01 |
Matrix | |||||
Brine (n = 8) | 42.38 ± 4.14 | 1.23 ± 0.19 | 2.11 ± 0.09 | 0.78 ± 0.04 | 0.95 ± 0.00 |
Fruit (n = 7) | 46.00 ± 11.28 | 1.20 ± 0.22 | 2.07 ± 0.35 | 0.75 ± 0.12 | 0.95 ± 0.01 |
Fungi Population | |||||
Time | Observed | FaithPD | Shannon | Simpson | Clonality |
T0 (n = 4) | 35.50 ± 1.91 | 2.08 ± 0.10 | 2.05 ± 0.09 | 0.70 ± 0.04 | 0.94 ± 0.00 |
T1 (n = 3) | 34.67 ± 1.53 | 2.01 ± 0.02 | 2.02 ± 0.04 | 0.70 ± 0.01 | 0.94 ± 0.00 |
T2 (n = 3) | 33.25 ± 3.40 | 2.07 ± 0.18 | 2.03 ± 0.15 | 0.70 ± 0.05 | 0.94 ± 0.00 |
T3 (n = 4) | 32.00 ± 2.00 | 1.87 ± 0.22 | 2.29 ± 0.30 | 0.78 ± 0.09 | 0.93 ± 0.01 |
Matrix | |||||
Brine (n = 8) | 33.63 ± 2.33 | 1.96 ± 0.14 | 2.08 ± 0.07 | 0.71 ± 0.02 | 0.94 ± 0.00 |
Fruit (n = 6) | 34.33 ± 2.94 | 2.09 ± 0.15 | 2.11 ± 0.28 | 0.72 ± 0.09 | 0.94 ± 0.01 |
Taxonomic Unit/Sample | * B-T0 | B-T1 | B-T2 | B-T3 | F-T0 | F-T1 | F-T2 | F-T3 |
---|---|---|---|---|---|---|---|---|
Lactiplantibacillus | 58.27 | 66.83 | 60.47 | 74.65 | 43.23 | 47.55 | 64.12 | 62.33 |
Pediococcus | 34.53 | 26.07 | 32.03 | 20.04 | 27.41 | 46.43 | 25.19 | 29.53 |
Celerinatantimonas | 4.77 | 5.05 | 5.07 | 3.68 | 27.89 | 3.91 | 8.62 | 5.23 |
Unassigned taxa | 0.23 | 0.11 | 0.19 | 0.15 | 0.09 | 0.12 | 0.19 | 0.19 |
Salinicola | 0.17 | 0.09 | 0.19 | 0.16 | 0.00 | 0.13 | 0.18 | 0.23 |
Alkalibacterium | 0.17 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
Marinilactibacillus | 0.00 | 0.14 | 0.08 | 0.08 | 0.06 | 0.17 | 0.15 | 0.19 |
Bacillaceae family | 0.00 | 0.00 | 0.12 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
Enterobacter | 0.00 | 0.00 | 0.00 | 0.00 | 0.18 | 0.00 | 0.00 | 0.00 |
Pseudomonas | 0.00 | 0.00 | 0.00 | 0.00 | 0.12 | 0.00 | 0.00 | 0.00 |
Suttonella | 0.00 | 0.00 | 0.06 | 0.08 | 0.00 | 0.00 | 0.14 | 0.17 |
Carnimonas | 0.00 | 0.00 | 0.00 | 0.00 | 0.11 | 0.00 | 0.00 | 0.00 |
Lactobacillaceae family | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.15 | 0.00 | 0.00 |
Genus/Sample | * B-T0 | B-T1 | B-T2 | B-T3 | F-T0 | F-T1 | F-T2 | F-T3 |
---|---|---|---|---|---|---|---|---|
Citeromyces | 53.63 | 56.46 | 56.05 | 53.95 | 57.95 | 57.63 | 56.88 | 19.25 |
Unassigned taxa | 27.24 | 27.06 | 26.13 | 34.53 | 24.03 | 24.53 | 24.14 | 32.01 |
Candida | 10.31 | 9.17 | 9.92 | 6.36 | 10.17 | 10.01 | 10.17 | 29.20 |
Penicillium | 7.89 | 6.57 | 7.07 | 4.79 | 7.00 | 6.46 | 7.76 | 17.01 |
Wickerhamomyces | 0.52 | 0.31 | 0.46 | 0.18 | 0.48 | 0.53 | 0.45 | 1.86 |
Aureobasidium | 0.23 | 0.18 | 0.21 | 0.12 | 0.22 | 0.21 | 0.28 | 0.00 |
Debaryomyces | 0.18 | 0.08 | 0.16 | 0.08 | 0.16 | 0.17 | 0.19 | 0.46 |
Cladosporium | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.13 | 0.00 |
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López-García, E.; Benítez-Cabello, A.; Ramiro-García, J.; Romero-Gil, V.; Rodríguez-Gómez, F.; Arroyo-López, F.N. New Insights into Microbial Diversity of the Traditional Packed Table Olives Aloreña de Málaga through Metataxonomic Analysis. Microorganisms 2021, 9, 561. https://doi.org/10.3390/microorganisms9030561
López-García E, Benítez-Cabello A, Ramiro-García J, Romero-Gil V, Rodríguez-Gómez F, Arroyo-López FN. New Insights into Microbial Diversity of the Traditional Packed Table Olives Aloreña de Málaga through Metataxonomic Analysis. Microorganisms. 2021; 9(3):561. https://doi.org/10.3390/microorganisms9030561
Chicago/Turabian StyleLópez-García, Elio, Antonio Benítez-Cabello, Javier Ramiro-García, Verónica Romero-Gil, Francisco Rodríguez-Gómez, and Francisco Noé Arroyo-López. 2021. "New Insights into Microbial Diversity of the Traditional Packed Table Olives Aloreña de Málaga through Metataxonomic Analysis" Microorganisms 9, no. 3: 561. https://doi.org/10.3390/microorganisms9030561