Developmental Alterations of Colonic microRNA Profiles Imply Potential Biological Functions in Kid Goats
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Animals and Colon Tissue Collection
2.2. RNA Isolation, Library Construction and Sequencing
2.3. miRNA Data Analyses
2.4. qPCR Validation of miRNA
2.5. Statistical Analyses
3. Results
3.1. miRNAs Expression Profile in the Colon Tissues
3.2. Pathway Analyses of the DEmiRNAs and HEmiRNAs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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miRNA | Sequences | D10_1 (norm) | D10_2 (norm) | D10_3 (norm) | D10_4 (norm) | D10_5 (norm) | D25_1 (norm) | D25_2 (norm) | D25_3 (norm) | D25_4 (norm) | D25_5 (norm) | D31_1 (norm) | D31_2 (norm) | D31_3 (norm) | D31_4 (norm) | D31_5 (norm) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
chi-miR-145-5p | GTCCAGTTTTCCCAGGAATCCCT | 413,928.08 | 273,737.54 | 305,373.85 | 259,564.08 | 158,024.67 | 793,964.42 | 638,968.89 | 541,464.83 | 631,723.29 | 709,422.09 | 752,564.55 | 708,834.75 | 619,420.59 | 683,159.93 | 806,093.4 |
chi-miR-150 | TCTCCCAACCCTTGTACCAGTG | 7238.49 | 7862.5 | 10,018.71 | 5326.91 | 3185.05 | 7577.22 | 4807.91 | 8794.09 | 8113.97 | 11,637.64 | 8922.71 | 9453.68 | 18,551.5 | 17,071.68 | 17,578.87 |
chi-miR-493-5p | TTGTACATGGTAGGCTTTCATT | 4999.65 | 7253.76 | 6637.88 | 4949.41 | 4818.39 | 2541.94 | 3025.21 | 3008.33 | 3475.73 | 1959.88 | 2622.32 | 3930.38 | 3009.98 | 2527.48 | 2423.76 |
chi-let-7a-5p | TGAGGTAGTAGGTTGTGTGGTT | 273,674.8 | 308,517.33 | 296,359.97 | 317,003.02 | 330,373.78 | 311,181.55 | 521,287.55 | 306,841.98 | 352,508.62 | 303,980.85 | 399,522.55 | 392,244.07 | 339,988.32 | 255,973.03 | 245,615.7 |
chi-let-7b-5p | TGAGGTAGTAGGTTGTGTGGTT | 176,240.7 | 173,168.72 | 187,498.46 | 222,957.97 | 151,793.56 | 227,617.78 | 245,458.22 | 194,446.03 | 201,309.73 | 256,727.74 | 242,442.15 | 267,225.59 | 239,427.19 | 275,758.49 | 21,3294.5 |
chi-let-7c-5p | TGAGGTAGTAGGTTGTATGGTT | 19,998.78 | 19,328.18 | 22,494.28 | 25,363.75 | 15,374.01 | 17,784.34 | 27,865.21 | 14,034.72 | 20,558.67 | 15,736.86 | 22,688.46 | 29,583.58 | 24,529.86 | 19,915.93 | 15,916.33 |
chi-let-7d-5p | AGAGGTAGTAGGTTGCATAGTT | 17,727.73 | 20,104.59 | 18,721.97 | 21,742.38 | 19,750.87 | 19,055.95 | 18,598.87 | 19,642.49 | 19,593.06 | 21,274.85 | 22,562.2 | 20,098.75 | 19,962.66 | 19,492.2 | 16,406.29 |
chi-let-7d-3p | CTATACGACCTGCTGCCTTTCT | 4844.61 | 7089.73 | 6341.19 | 6762.08 | 5883.03 | 3801.47 | 14,170.87 | 6507.76 | 5145.36 | 9214.98 | 5528.73 | 7017.13 | 6903.8 | 7994.49 | 5557.47 |
chi-let-7e-5p | TGAGGTAGGAGGTTGTATAGTT | 32,213.65 | 40,306.38 | 40,948.79 | 39,775.16 | 33,621.36 | 32,349.5 | 34,276.18 | 31,642.95 | 39,923.47 | 33,233.69 | 41,543.94 | 50,541.23 | 36,741.77 | 29,093.51 | 27,127.68 |
chi-let-7f-5p | TGAGGTAGTAGATTGTATAGTT | 31,245.66 | 31,577.41 | 24,545.72 | 22,108.92 | 40,557.5 | 31,878.85 | 68,705.39 | 37,339.47 | 31,208.06 | 27,579.35 | 28,455.72 | 29,908.99 | 24,931.13 | 17,577.23 | 23,287.28 |
chi-let-7g-5p | TGAGGTAGTAGTTTGTACAGTT | 20,827.40 | 27,945.82 | 19,867.21 | 20,364.65 | 31,014.77 | 20,261.49 | 53,386.92 | 22,753.72 | 24,312.68 | 20,075.23 | 24,907.09 | 27,355.42 | 20,522.19 | 15,088.47 | 16,710.72 |
chi-let-7i-5p | TGAGGTAGTAGTTTGTGCTGTT | 32,712.21 | 31,512.86 | 26,314.27 | 25,278.73 | 34,437.59 | 31,221.13 | 79,115.93 | 37,108.08 | 29,293.7 | 28,501.77 | 28,455.99 | 31,761.57 | 27,101.97 | 22,835.44 | 20,823.23 |
chi-miR-29a-5p | ACTGATTTCTTTTGGTGTTCAGAGT | 6.20 | 6.38 | 4.92 | 0.00 | 11.53 | 30.49 | 0.00 | 26.32 | 14.12 | 0.00 | 47.35 | 12.27 | 9.38 | 28.05 | 28.19 |
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Yan, Q.; Tian, L.; Chen, W.; Kang, J.; Tang, S.; Tan, Z. Developmental Alterations of Colonic microRNA Profiles Imply Potential Biological Functions in Kid Goats. Animals 2022, 12, 1533. https://doi.org/10.3390/ani12121533
Yan Q, Tian L, Chen W, Kang J, Tang S, Tan Z. Developmental Alterations of Colonic microRNA Profiles Imply Potential Biological Functions in Kid Goats. Animals. 2022; 12(12):1533. https://doi.org/10.3390/ani12121533
Chicago/Turabian StyleYan, Qiongxian, Lina Tian, Wenxun Chen, Jinhe Kang, Shaoxun Tang, and Zhiliang Tan. 2022. "Developmental Alterations of Colonic microRNA Profiles Imply Potential Biological Functions in Kid Goats" Animals 12, no. 12: 1533. https://doi.org/10.3390/ani12121533