Heat-Killed Lactobacillus acidophilus Promotes Growth by Modulating the Gut Microbiota Composition and Fecal Metabolites of Piglets
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animal Ethics
2.2. Preparation of Heat-Killed Lactobacillus acidophilus IFFI 6005
2.3. Animal and Experimental Design
2.4. Animal Administrations
2.5. Test Indicator Determination
2.6. Fecal Sample Collection and Index Determination
2.6.1. Fecal Sample Collection
2.6.2. Illumina Sequencing
2.6.3. Determination and Analysis of the Metabolome
2.7. Integration Analysis between Microbiota and Metabolome
2.8. Statistical Analysis
3. Results
3.1. Preparation of Heat-Killed Lactobacillus acidophilus IFFI 6005
3.2. Growth Performance and Diarrhea Rate
3.3. Analysis of the Intestinal Microbiota
3.3.1. Analysis of Alpha Diversity in Intestinal Microorganisms
3.3.2. Identification of Target Microorganisms by Differential Analysis
3.4. Analysis of Fecal Metabolites
3.5. Association Analysis of Differential Microorganisms and Metabolites
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Items | NC | PC | LA | HKLA | SEM | p Value |
---|---|---|---|---|---|---|
BW1 (kg) | 14.28 | 14.36 | 14.63 | 14.25 | 0.37 | 0.843 |
BW30 (kg) | 35.75 a | 37.55 b | 36.25 a | 38.74 b | 0.69 | 0.045 |
ADG (g) | 715.67 a | 773.00 bc | 720.67 a | 816.33 c | 13.36 | 0.025 |
ADFI (g) | 1090.76 a | 1051.28 a | 1095.42 a | 1142.86 b | 18.64 | 0.0659 |
F:G | 1.52 a | 1.36 b | 1.52 a | 1.40 b | 0.02 | 0.017 |
Diarrhoea rate (%) | 5.83 a | 3.33 b | 5.14 a | 2.64 b | 0.15 | 0.021 |
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Miao, H.; Liang, J.; Lan, G.; Wu, Q.; Huang, Z. Heat-Killed Lactobacillus acidophilus Promotes Growth by Modulating the Gut Microbiota Composition and Fecal Metabolites of Piglets. Animals 2024, 14, 2528. https://doi.org/10.3390/ani14172528
Miao H, Liang J, Lan G, Wu Q, Huang Z. Heat-Killed Lactobacillus acidophilus Promotes Growth by Modulating the Gut Microbiota Composition and Fecal Metabolites of Piglets. Animals. 2024; 14(17):2528. https://doi.org/10.3390/ani14172528
Chicago/Turabian StyleMiao, Huabiao, Jing Liang, Ganqiu Lan, Qian Wu, and Zunxi Huang. 2024. "Heat-Killed Lactobacillus acidophilus Promotes Growth by Modulating the Gut Microbiota Composition and Fecal Metabolites of Piglets" Animals 14, no. 17: 2528. https://doi.org/10.3390/ani14172528