Baicalin Enhances Chemosensitivity to Doxorubicin in Breast Cancer Cells via Upregulation of Oxidative Stress-Mediated Mitochondria-Dependent Apoptosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Culture
2.3. Investigation of Colony Formation
2.4. Determination of Cell Viability, Cytotoxicity, and Apoptosis
2.5. Measurement of ROS Production and Antioxidant Enzyme Activity
2.6. Measurement of Calcium Concentration
2.7. Immunoblotting
2.8. Measurement of Active Caspase-3
2.9. Measurement of Mitochondrial Membrane Potential (△Ψm)
2.10. Statistical Analyses
3. Results
3.1. Baicalin Increased Dox-Impaired Cell Viability and Reduced Cell Survival under Dox Treatment in MDA-MB-231 and MCF-7 Breast Cancer Cells
3.2. Baicalin Enhanced Dox-Induced Oxidative Stress in MDA-MB-231 and MCF-7 Breast Cancer Cells
3.3. Baicalin Increased Intracellular Calcium Concentration and Impairment of Mitochondrial Membrane Potential under Dox Treatment
3.4. Baicalin Promoted Apoptosis in Breast Cancer Cells with Dox Treatment and Increased Chemoselectivity in Dox-Resistant Breast Cancer Cells, but Protected Cardiomyoblast Cells from Dox-Induced Apoptosis
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Lin, M.-Y.; Cheng, W.-T.; Cheng, H.-C.; Chou, W.-C.; Chen, H.-I.; Ou, H.-C.; Tsai, K.-L. Baicalin Enhances Chemosensitivity to Doxorubicin in Breast Cancer Cells via Upregulation of Oxidative Stress-Mediated Mitochondria-Dependent Apoptosis. Antioxidants 2021, 10, 1506. https://doi.org/10.3390/antiox10101506
Lin M-Y, Cheng W-T, Cheng H-C, Chou W-C, Chen H-I, Ou H-C, Tsai K-L. Baicalin Enhances Chemosensitivity to Doxorubicin in Breast Cancer Cells via Upregulation of Oxidative Stress-Mediated Mitochondria-Dependent Apoptosis. Antioxidants. 2021; 10(10):1506. https://doi.org/10.3390/antiox10101506
Chicago/Turabian StyleLin, Mei-Yi, Wan-Ting Cheng, Hui-Ching Cheng, Wan-Ching Chou, Hsiu-I Chen, Hsiu-Chung Ou, and Kun-Ling Tsai. 2021. "Baicalin Enhances Chemosensitivity to Doxorubicin in Breast Cancer Cells via Upregulation of Oxidative Stress-Mediated Mitochondria-Dependent Apoptosis" Antioxidants 10, no. 10: 1506. https://doi.org/10.3390/antiox10101506