Colorimetric Determination of the Activity of Starch-Debranching Enzyme via Modified Tollens’ Reaction
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Debranching of Waxy Maize Starch
2.3. Separation of SCG From the Debranching Reaction
2.4. Measurement of Debranching Enzyme Activity
2.5. Characterization of AgNPs Synthesized During Modified Tollens’ Reaction
3. Results and Discussion
3.1. Synthesis of AgNPs Using Debranched Starch via Modified Tollens’ Reaction
3.2. Characterization of AgNPs Synthesized by Modified Tollens’ Reaction
3.3. Quantification of Debranched SCGs through Modified Tollens’ Reaction
3.4. Investigation of the Debranching Reaction in a Real Sample
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Luo, K.; Kim, N.-g.; You, S.-M.; Kim, Y.-R. Colorimetric Determination of the Activity of Starch-Debranching Enzyme via Modified Tollens’ Reaction. Nanomaterials 2019, 9, 1291. https://doi.org/10.3390/nano9091291
Luo K, Kim N-g, You S-M, Kim Y-R. Colorimetric Determination of the Activity of Starch-Debranching Enzyme via Modified Tollens’ Reaction. Nanomaterials. 2019; 9(9):1291. https://doi.org/10.3390/nano9091291
Chicago/Turabian StyleLuo, Ke, Nack-geun Kim, Sang-Mook You, and Young-Rok Kim. 2019. "Colorimetric Determination of the Activity of Starch-Debranching Enzyme via Modified Tollens’ Reaction" Nanomaterials 9, no. 9: 1291. https://doi.org/10.3390/nano9091291