The A226D Mutation of OmpC Leads to Increased Susceptibility to β-Lactam Antibiotics in Escherichia coli
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Search for E. coli Strains with OmpC A226D Mutation
2.2. Mutation of ompC
2.3. Construction of ompC-Containing Strains
2.4. Drug Susceptibility Test
2.5. Survival Assays
2.6. Detection of ompC Expression Level
2.7. Molecular Dynamics and Docking
2.8. Statistics
3. Results
3.1. Identification of an ompC A226D Mutation
3.2. OmpC A226D Mutation Leads to Increase of β-Lactam Susceptibility
3.3. Structural Changes Resulting from A226D Mutation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pores | A226D | WT |
---|---|---|
AMP | ||
Pore 1 | −8.63 | −8.8 |
Pore 2 | −7.96 | −8.56 |
Pore 3 | −8.93 | −8.03 |
PIP | ||
Pore 1 | −7.17 | −9.69 |
Pore 2 | −7.77 | −11.31 |
Pore 3 | −6.64 | −9.64 |
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Zhu, J.; Guo, P.; Zheng, Y.; Xiang, S.; Zhao, Y.; Liu, X.; Fu, C.; Zhang, Y.; Xu, H.; Li, L.; et al. The A226D Mutation of OmpC Leads to Increased Susceptibility to β-Lactam Antibiotics in Escherichia coli. Biology 2024, 13, 600. https://doi.org/10.3390/biology13080600
Zhu J, Guo P, Zheng Y, Xiang S, Zhao Y, Liu X, Fu C, Zhang Y, Xu H, Li L, et al. The A226D Mutation of OmpC Leads to Increased Susceptibility to β-Lactam Antibiotics in Escherichia coli. Biology. 2024; 13(8):600. https://doi.org/10.3390/biology13080600
Chicago/Turabian StyleZhu, Jiaming, Peng Guo, Yuting Zheng, Shiqing Xiang, Yang Zhao, Xinyu Liu, Chengzhang Fu, Youming Zhang, Hai Xu, Ling Li, and et al. 2024. "The A226D Mutation of OmpC Leads to Increased Susceptibility to β-Lactam Antibiotics in Escherichia coli" Biology 13, no. 8: 600. https://doi.org/10.3390/biology13080600