Comparative Analysis of Angora Rabbit Colostrum and Mature Milk Using Quantitative Proteomics
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Milk Sample Collection and Preparation
2.2. Total Protein Extraction
2.3. Trypsin Digestion
2.4. Data-Dependent Acquisition (DDA) and DIA Analyses
2.5. Protein Identification and Quantification
2.6. Bioinformatics and Statistical Analysis
2.7. Parallel Reaction Monitoring (PRM) Validation
3. Results
3.1. Daily Milk Yields and Litter Sizes Alive
3.2. Component Analysis of Proteins from Colostrum and Mature Milk
3.3. Characterization of DAPs
3.4. GO Annotation and KEGG Pathway Analyses
3.5. PPI Network and Module Analysis
3.6. Validation of DAPs by PRM
4. Discussion
4.1. Daily Milk Production and Associated Traits
4.2. Screening of Candidate Proteins in Colostrum and Mature Milk
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Huang, D.; Wang, Y.; Ding, H.; Zhao, H. Comparative Analysis of Angora Rabbit Colostrum and Mature Milk Using Quantitative Proteomics. Biology 2024, 13, 634. https://doi.org/10.3390/biology13080634
Huang D, Wang Y, Ding H, Zhao H. Comparative Analysis of Angora Rabbit Colostrum and Mature Milk Using Quantitative Proteomics. Biology. 2024; 13(8):634. https://doi.org/10.3390/biology13080634
Chicago/Turabian StyleHuang, Dongwei, Yuanlang Wang, Haisheng Ding, and Huiling Zhao. 2024. "Comparative Analysis of Angora Rabbit Colostrum and Mature Milk Using Quantitative Proteomics" Biology 13, no. 8: 634. https://doi.org/10.3390/biology13080634