Carbidopa Alters Tryptophan Metabolism in Breast Cancer and Melanoma Cells Leading to the Formation of Indole-3-Acetonitrile, a Pro-Proliferative Metabolite
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Viability Assays
2.2. LC-MS Analysis
2.3. HPLC Analysis
2.4. Statistical Analysis
3. Results
3.1. Involvement of Trp Metabolites in Cancer
3.2. Exposure of A375 and MCF-7 to Carbidopa
3.3. IAN Increases the Viability of Tumour Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Duarte, D.; Amaro, F.; Silva, I.; Silva, D.; Fresco, P.; Oliveira, J.C.; Reguengo, H.; Gonçalves, J.; Vale, N. Carbidopa Alters Tryptophan Metabolism in Breast Cancer and Melanoma Cells Leading to the Formation of Indole-3-Acetonitrile, a Pro-Proliferative Metabolite. Biomolecules 2019, 9, 409. https://doi.org/10.3390/biom9090409
Duarte D, Amaro F, Silva I, Silva D, Fresco P, Oliveira JC, Reguengo H, Gonçalves J, Vale N. Carbidopa Alters Tryptophan Metabolism in Breast Cancer and Melanoma Cells Leading to the Formation of Indole-3-Acetonitrile, a Pro-Proliferative Metabolite. Biomolecules. 2019; 9(9):409. https://doi.org/10.3390/biom9090409
Chicago/Turabian StyleDuarte, Diana, Filipa Amaro, Isabel Silva, Dany Silva, Paula Fresco, José C. Oliveira, Henrique Reguengo, Jorge Gonçalves, and Nuno Vale. 2019. "Carbidopa Alters Tryptophan Metabolism in Breast Cancer and Melanoma Cells Leading to the Formation of Indole-3-Acetonitrile, a Pro-Proliferative Metabolite" Biomolecules 9, no. 9: 409. https://doi.org/10.3390/biom9090409