Blood Vessel Patterning on Retinal Astrocytes Requires Endothelial Flt-1 (VEGFR-1)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mouse Husbandry
2.2. Inducible Cre Excision and Retina Tissue Processing
2.3. Quantitative Analysis of Retinal Vasculature
2.4. Statistical Analysis
3. Results
3.1. Induced Genetic Deletion of Flt-1 Leads to Sustained and Excessive Vascular Growth In Vivo
3.2. Conditional Flt-1 Ablation Disrupts Endothelial Sprouting in the Developing Retinal Vasculature.
3.3. Retinal Astrocyte Patterning Is Unaffected by the Conditional Loss of Flt-1
3.4. Mosaic flt-1 Expression at the Vascular Front Increases Endothelial Filopodial Extensions on Underlying Astrocytes
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Chappell, J.C.; Darden, J.; Payne, L.B.; Fink, K.; Bautch, V.L. Blood Vessel Patterning on Retinal Astrocytes Requires Endothelial Flt-1 (VEGFR-1). J. Dev. Biol. 2019, 7, 18. https://doi.org/10.3390/jdb7030018
Chappell JC, Darden J, Payne LB, Fink K, Bautch VL. Blood Vessel Patterning on Retinal Astrocytes Requires Endothelial Flt-1 (VEGFR-1). Journal of Developmental Biology. 2019; 7(3):18. https://doi.org/10.3390/jdb7030018
Chicago/Turabian StyleChappell, John C., Jordan Darden, Laura Beth Payne, Kathryn Fink, and Victoria L. Bautch. 2019. "Blood Vessel Patterning on Retinal Astrocytes Requires Endothelial Flt-1 (VEGFR-1)" Journal of Developmental Biology 7, no. 3: 18. https://doi.org/10.3390/jdb7030018