Tracking of Zinc Ferrite Nanoparticle Effects on Pea (Pisum sativum L.) Plant Growth, Pigments, Mineral Content and Arbuscular Mycorrhizal Colonization
Abstract
:1. Introduction
2. Results
2.1. X-ray Diffraction (XRD) Analysis
2.2. Magnetic Hysteresis (M-H) Measurements
2.3. Transmission Electron Microscope (TEM)
2.4. Effects of ZnFe2O4 NPs on Plant Growth Traits
2.5. AM Fungal Colonization Rate
2.6. Photosynthetic Pigments Content
2.7. Fe and Zn Content and Their Translocation in Plant Tissues
3. Discussion
4. Materials and Methods
4.1. Synthesis of ZnFe2O4 NPs
4.2. Nanoparticle Characterization
4.3. Preparation of ZnFe2O4 NPs Suspension
4.4. Soil, Seeds, Pot Culture and Growth Condition
4.5. Arbuscular Mycorrhizal (AM) Fungal Inoculation, ZnFe2O4 NPs Treatment and Sample Collection
4.6. Measurements
4.6.1. Growth Traits
4.6.2. AM Fungal Colonization Percentage
4.6.3. Photosynthetic Pigment Contents
4.6.4. Fe and Zn Contents and Translocation Factor (TF)
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Variables | AM | ZnFe2O4 NPs | AM+ ZnFe2O4 NPs |
---|---|---|---|
Shoot FW | 40.47 * | 14.39 * | 0.167 ns |
Root FW | 81.49 * | 10.56 * | 6.39 * |
Shoot DW | 39.85 * | 36.46 * | 11.81 ns |
Root DW | 65.56 * | 43.86 * | 1.06 ns |
R/S ratio | 26.02 * | 2.89 ns | 32.12 * |
Chlorophyll a | 4.269 ns | 2.038 ns | 0.559 ns |
Chlorophyll b | 50.05 * | 8.788 * | 15.658 * |
Carotenoids | 22.16 * | 4.93 ns | 9.48 * |
Total Chlorophyll | 13.3 * | 5.886 * | 0.079 ns |
Total pigments | 7.63 * | 5.82 * | 2.16 ns |
Shoot Zn conc | 7.956 * | 138.8 * | 0.213 ns |
Root Zn conc | 6.74 * | 54.56 * | 0.207 ns |
Total Zn conc | 7.49 * | 101.15 * | 0.211 ns |
Treatment | Chlorophyll a | Chlorophyll b | Carotenoids | Total Chlorophyll | Total Pigments |
---|---|---|---|---|---|
(mg g−1 FW) | (mg g−1 FW) | (mg g−1 FW) | (mg g−1 FW) | (mg g−1 FW) | |
Control | 1.441 ± 0.076 b | 0.812 ± 0.043 bc | 0.297 ± 0.016 b | 2.252 ± 0.12 b | 2.549 ± 0.135 b |
AM | 1.497 ± 0.079 ab | 0.976 ± 0.052 b | 0.270 ± 0.014 b | 2.473 ± 0.13 b | 2.743 ± 0.145 b |
ZnFe2O4 NPs | 1.549 ± 0.082 ab | 0.759 ± 0.04 c | 0.382 ± 0.02 a | 2.309 ± 0.12 b | 2.691 ± 0.142 b |
AM+ ZnFe2O4 NPs | 1.729 ± 0.091 a | 1.342 ± 0.071 a | 0.256 ± 0.014 b | 3.071 ± 0.16 a | 3.327 ± 0.176 a |
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Abdelhameed, R.E.; Abu-Elsaad, N.I.; Abdel Latef, A.A.H.; Metwally, R.A. Tracking of Zinc Ferrite Nanoparticle Effects on Pea (Pisum sativum L.) Plant Growth, Pigments, Mineral Content and Arbuscular Mycorrhizal Colonization. Plants 2021, 10, 583. https://doi.org/10.3390/plants10030583
Abdelhameed RE, Abu-Elsaad NI, Abdel Latef AAH, Metwally RA. Tracking of Zinc Ferrite Nanoparticle Effects on Pea (Pisum sativum L.) Plant Growth, Pigments, Mineral Content and Arbuscular Mycorrhizal Colonization. Plants. 2021; 10(3):583. https://doi.org/10.3390/plants10030583
Chicago/Turabian StyleAbdelhameed, Reda E., Nagwa I. Abu-Elsaad, Arafat Abdel Hamed Abdel Latef, and Rabab A. Metwally. 2021. "Tracking of Zinc Ferrite Nanoparticle Effects on Pea (Pisum sativum L.) Plant Growth, Pigments, Mineral Content and Arbuscular Mycorrhizal Colonization" Plants 10, no. 3: 583. https://doi.org/10.3390/plants10030583