Foliar Spray of Alpha-Tocopherol Modulates Antioxidant Potential of Okra Fruit under Salt Stress
Abstract
:1. Introduction
2. Results
3. Discussion
4. Material and Methods
4.1. Estimation of Enzymatic Antioxidants
4.2. Estimation of Non-Enzymatic Antioxidants
4.3. Determination of ROS
4.4. Nutrients Analysis for Na+, K+ and Ca2+
4.5. Yield
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Temam, N.; Mohammed, W.; Aklilu, S.; Belayneh, A.H.; Gebremedhin, K.G.; Egziabher, Y.G. Variability Assessment of Okra (Abelmoschus esculentus (L.) Moench) Genotypes Based on Their Qualitative Traits. Int. J. Agron. 2021, 2021, 6678561. [Google Scholar] [CrossRef]
- Haruna, S.; Aliyu, B.S.; Bala, A. Plant gum exudates (Karau) and mucilages, their biological sources, properties, uses and potential applications: A review. Bayero J. Pure Appl. Sci. 2016, 9, 159–165. [Google Scholar] [CrossRef] [Green Version]
- Mushtaq, N.U.; Saleem, S.; Rasool, A.; Shah, W.H.; Hakeem, K.R.; Rehman, R.U. Salt Stress Threshold in Millets: Perspective on Cultivation on Marginal Lands for Biomass. Phyton 2020, 90, 51. [Google Scholar] [CrossRef]
- Mushtaq, Z.; Faizan, S.; Gulzar, B. Salt stress, its impacts on plants and the strategies plants are employing against it: A review. J. Appl. Biol. Biotechnol. 2020, 8, 81–91. [Google Scholar]
- Khan, A.; Khan, A.L.; Muneer, S.; Kim, Y.-H.; Al-Rawahi, A.; Al-Harrasi, A. Silicon and salinity: Crosstalk in crop-mediated stress tolerance mechanisms. Front. Plant Sci. 2019, 10, 1429. [Google Scholar] [CrossRef] [Green Version]
- Naveed, M.; Sajid, H.; Mustafa, A.; Niamat, B.; Ahmad, Z.; Yaseen, M.; Kamran, M.; Rafique, M.; Ahmar, S.; Chen, J.-T. Alleviation of salinity-induced oxidative stress, improvement in growth, physiology and mineral nutrition of canola (Brassica napus L.) through calcium-fortified composted animal manure. Sustainability 2020, 12, 846. [Google Scholar]
- Moles, T.M.; de Brito Francisco, R.; Mariotti, L.; Pompeiano, A.; Lupini, A.; Incrocci, L.; Carmassi, G.; Scartazza, A.; Pistelli, L.; Guglielminetti, L. Salinity in autumn-winter season and fruit quality of tomato landraces. Front. Plant Sci. 2019, 10, 1078. [Google Scholar] [CrossRef]
- Maria, N.; Muhammad, S.; Abdul, W.; Waraich, E.A. Seed priming with alpha tocopherol improves morpho-physiological attributes of okra under saline conditions. Int. J. Agric. Biol. 2018, 20, 2647–2654. [Google Scholar]
- Sadiq, M.; Akram, N.A.; Ashraf, M.; Al-Qurainy, F.; Ahmad, P. Alpha-tocopherol-induced regulation of growth and metabolism in plants under non-stress and stress conditions. J. Plant Growth Regul. 2019, 38, 1325–1340. [Google Scholar]
- Soltabayeva, A.; Ongaltay, A.; Omondi, J.O.; Srivastava, S. Morphological, Physiological and Molecular Markers for Salt-Stressed Plants. Plants 2021, 10, 243. [Google Scholar] [CrossRef] [PubMed]
- Demidchik, V. Mechanisms of oxidative stress in plants: From classical chemistry to cell biology. Environ. Exp. Bot. 2015, 109, 212–228. [Google Scholar] [CrossRef]
- Kumar, S.; Li, G.; Yang, J.; Huang, X.; Ji, Q.; Zhou, K.; Khan, S.; Ke, W.; Hou, H. Investigation of an Antioxidative System for Salinity Tolerance in Oenanthe javanica. Antioxidants 2020, 9, 940. [Google Scholar] [CrossRef]
- Surówka, E.; Potocka, I.; Dziurka, M.; Wróbel-Marek, J.; Kurczyńska, E.; Żur, I.; Maksymowicz, A.; Gajewska, E.; Miszalski, Z. Tocopherols mutual balance is a key player for maintaining Arabidopsis thaliana growth under salt stress. Plant Physiol. Biochem. 2020, 156, 369–383. [Google Scholar] [CrossRef] [PubMed]
- Semida, W.; Taha, R.; Abdelhamid, M.; Rady, M. Foliar-applied α-tocopherol enhances salt-tolerance in Vicia faba L. plants grown under saline conditions. S. Afr. J. Bot. 2014, 95, 24–31. [Google Scholar] [CrossRef] [Green Version]
- Fritsche, S.; Wang, X.; Jung, C. Recent advances in our understanding of tocopherol biosynthesis in plants: An overview of key genes, functions, and breeding of vitamin E improved crops. Antioxidants 2017, 6, 99. [Google Scholar] [CrossRef] [Green Version]
- Bose, J.; Rodrigo-Moreno, A.; Shabala, S. ROS homeostasis in halophytes in the context of salinity stress tolerance. J. Exp. Bot. 2014, 65, 1241–1257. [Google Scholar] [CrossRef] [PubMed]
- Laxa, M.; Liebthal, M.; Telman, W.; Chibani, K.; Dietz, K.-J. The role of the plant antioxidant system in drought tolerance. Antioxidants 2019, 8, 94. [Google Scholar] [CrossRef] [Green Version]
- Luis, A.; Corpas, F.J.; López-Huertas, E.; Palma, J.M. Plant superoxide dismutases: Function under abiotic stress conditions. In Antioxidants and Antioxidant Enzymes in Higher Plants; Springer: Berlin/Heidelberg, Germany, 2018; pp. 1–26. [Google Scholar]
- Dubey, R.S. Photosynthesis in plants under stressful conditions. In Handbook of Photosynthesis; CRC Press: Boca Raton, FL, USA, 2018; pp. 629–649. [Google Scholar]
- Singh, D. Stress Physiology; New Age International (P) limited, Publishers: New Dehli, India, 2003. [Google Scholar]
- Stefanov, M.; Biswal, A.; Misra, M.; Misra, A.; Apostolova, E. Responses of Photosynthetic Apparatus to Salt Stress: Structure, Function, and Protection. In Handbook of Plant and Crop Stress, 4th ed.; CRC Press: Boca Raton, FL, USA, 2019; pp. 233–250. [Google Scholar]
- Muhammad, I.; Shalmani, A.; Ali, M.; Yang, Q.-H.; Ahmad, H.; Li, F.B. Mechanisms regulating the dynamics of photosynthesis under abiotic stresses. Front. Plant Sci. 2021, 11, 2310. [Google Scholar] [CrossRef] [PubMed]
- Wang, W.-B.; Kim, Y.-H.; Lee, H.-S.; Kim, K.-Y.; Deng, X.-P.; Kwak, S.-S. Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiol. Biochem. 2009, 47, 570–577. [Google Scholar] [CrossRef]
- Rady, M.; Sadak, M.S.; El-Bassiouny, H.; El-Monem, A. Alleviation the adverse effects of salinity stress in sunflower cultivars using nicotinamide and α-tocopherol. Aust. J. Basic Appl. Sci. 2011, 5, 342–355. [Google Scholar]
- Mehla, N.; Sindhi, V.; Josula, D.; Bisht, P.; Wani, S.H. An introduction to antioxidants and their roles in plant stress tolerance. In Reactive Oxygen Species and Antioxidant Systems in Plants: Role and Regulation under Abiotic Stress; Springer: Berlin/Heidelberg, Germany, 2017; pp. 1–23. [Google Scholar]
- Hasanuzzaman, M.; Nahar, K.; Fujita, M. Plant response to salt stress and role of exogenous protectants to mitigate salt-induced damages. In Ecophysiology and Responses of Plants under Salt Stress; Springer: Berlin/Heidelberg, Germany, 2013; pp. 25–87. [Google Scholar]
- Ali, Q.; Javed, M.T.; Noman, A.; Haider, M.Z.; Waseem, M.; Iqbal, N.; Waseem, M.; Shah, M.S.; Shahzad, F.; Perveen, R. Assessment of drought tolerance in mung bean cultivars/lines as depicted by the activities of germination enzymes, seedling’s antioxidative potential and nutrient acquisition. Arch. Agron. Soil Sci. 2018, 64, 84–102. [Google Scholar] [CrossRef]
- Abedi, T.; Pakniyat, H. Antioxidant enzymes changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.). Czech J. Genet. Plant Breed. 2010, 46, 27–34. [Google Scholar] [CrossRef]
- Szarka, A.; Tomasskovics, B.; Bánhegyi, G. The ascorbate-glutathione-α-tocopherol triad in abiotic stress response. Int. J. Mol. Sci. 2012, 13, 4458. [Google Scholar] [CrossRef] [Green Version]
- Li, J.; Jin, H. Regulation of brassinosteroid signaling. Trends Plant Sci. 2007, 12, 37–41. [Google Scholar] [CrossRef] [PubMed]
- Sakamoto, A.; Murata, N. The role of glycine betaine in the protection of plants from stress: Clues from transgenic plants. Plant Cell Environ. 2002, 25, 163–171. [Google Scholar] [CrossRef]
- Tavakkoli, E.; Rengasamy, P.; McDonald, G.K. High concentrations of Na+ and Cl– ions in soil solution have simultaneous detrimental effects on growth of faba bean under salinity stress. J. Exp. Bot. 2010, 61, 4449–4459. [Google Scholar] [CrossRef]
- Ashraf, M.; Harris, P.J. Photosynthesis under stressful environments: An overview. Photosynthetica 2013, 51, 163–190. [Google Scholar] [CrossRef]
- Farooq, M.; Gogoi, N.; Barthakur, S.; Baroowa, B.; Bharadwaj, N.; Alghamdi, S.S.; Siddique, K. Drought stress in grain legumes during reproduction and grain filling. J. Agron. Crop. Sci. 2017, 203, 81–102. [Google Scholar] [CrossRef]
- Wang, X.; Wang, G.; Guo, T.; Xing, Y.; Mo, F.; Wang, H.; Fan, J.; Zhang, F. Effects of plastic mulch and nitrogen fertilizer on the soil microbial community, enzymatic activity and yield performance in a dryland maize cropping system. Eur. J. Soil Sci. 2021, 72, 400–412. [Google Scholar] [CrossRef]
- Wang, X.; Fan, J.; Xing, Y.; Xu, G.; Wang, H.; Deng, J.; Wang, Y.; Zhang, F.; Li, P.; Li, Z. The effects of mulch and nitrogen fertilizer on the soil environment of crop plants. Adv. Agron. 2019, 153, 121–173. [Google Scholar]
- Chance, B.; Maehly, A. Assay of catalases and peroxidases. Methods Enzymol. 1955, 2, 764–775. [Google Scholar]
- Giannopolitis, C.N.; Ries, S.K. Superoxide dismutases: I. Occurrence in higher plants. Plant Physiol. 1977, 59, 309–314. [Google Scholar] [CrossRef]
- Carlberg, I.; Mannervik, B. Glutathione reductase. Methods Enzymol. 1985, 113, 484–490. [Google Scholar] [PubMed]
- Drapeau, G. Protease from Staphylococcus Aureus, Method of Enzymology 45b, L; Academic Press: New York, NY, USA, 1974. [Google Scholar]
- Ainsworth, E.A.; Gillespie, K.M. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nat. Protoc. 2007, 2, 875–877. [Google Scholar] [CrossRef] [PubMed]
- Mukherjee, S.; Choudhuri, M. Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol. Plant. 1983, 58, 166–170. [Google Scholar] [CrossRef]
- Grieve, C.; Grattan, S. Rapid assay for determination of water soluble quaternary ammonium compounds. Plant Soil 1983, 70, 303–307. [Google Scholar] [CrossRef]
- Bates, L.S.; Waldren, R.P.; Teare, I. Rapid determination of free proline for water-stress studies. Plant Soil 1973, 39, 205–207. [Google Scholar] [CrossRef]
- Alexieva, V.; Sergiev, I.; Mapelli, S.; Karanov, E. The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell Environ. 2001, 24, 1337–1344. [Google Scholar] [CrossRef]
- Dhindsa, R.S.; Plumb-Dhindsa, P.; Thorpe, T.A. Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J. Exp. Bot. 1981, 32, 93–101. [Google Scholar] [CrossRef]
- Wolf, B. A comprehensive system of leaf analyses and its use for diagnosing crop nutrient status. Commun. Soil Sci. Plant Anal. 1982, 13, 1035–1059. [Google Scholar] [CrossRef]
- Snedecor, G.W.; Cochran, W.G. Statistical Methods, 7th ed.; Iowa State University Press: Ames, IA, USA, 1980. [Google Scholar]
Source | df | CAT | POD | SOD | GPX | Protease | Phenolics | Ascorbic Acid |
---|---|---|---|---|---|---|---|---|
V | 1 | 264.06 ns | 0.266 ns | 119.52 ** | 690,058.34 *** | 28,532.84 *** | 1.22 *** | 1388.89 ns |
S | 1 | 15,314.06 *** | 0.548 ns | 54.83 ns | 306,132.59 *** | 8883.06 *** | 293,265,625 *** | 41,769.14 *** |
α-toc | 3 | 2143.22 ** | 0.515 ns | 16.29 ns | 279,930.76 *** | 607.43 ns | 1.32 *** | 4013.78 * |
V × S | 1 | 1501.56 ns | 0.523 ns | 47.23 ns | 290,599.16 *** | 70.84 ns | 3.94 *** | 28,110.11 *** |
V × α-toc | 3 | 489.06 ns | 0.065 ns | 21.94 ns | 18,401.16 ns | 48,183.32 *** | 6,912,135.4 ns | 3462.61 * |
S × α-toc | 3 | 205.72 ns | 0.997 ** | 6.42 ns | 4860.57 ns | 18,282.91 *** | 32,911,354 ** | 16,191.37 *** |
V × S × α-toc | 3 | 801.56 ns | 0.109 ns | 24.20 ns | 57,395.68 * | 7053.95 *** | 9,296,354.2 ns | 13,829.37 *** |
Error | 48 | 403.64 | 0.184 | 15.23 | 18,769.04 | 404.83 | 6,673,880.2 | 1109.76 |
Source | df | Proline | GB | H2O2 | MDA | Na+ | K+ | Ca2+ | cap/pl |
---|---|---|---|---|---|---|---|---|---|
V | 1 | 992.25 ns | 0.33 ns | 7284.92 * | 228.39 ns | 0.19 ns | 11.81 *** | 8.6 ** | 43.89 *** |
S | 1 | 10,905.3 ** | 316.19 ns | 3519.39 ns | 25,656.80 *** | 53.47 *** | 36.75 *** | 30.9 *** | 43.88 *** |
α-toc | 3 | 1983.47 ns | 1097.5 ** | 1344.55 ns | 5587.57 ** | 1.11 ns | 1.19 ns | 7.9 *** | 9.515 ** |
V × S | 1 | 2102.87 ns | 5.3 ns | 63.87 ns | 8050.72 ** | 18.59 *** | 1.72 ns | 3.7 * | 0.390 ns |
V × α-toc | 3 | 2973.15 * | 313.3 * | 840.67 ns | 12,923.24 *** | 6.34 *** | 0.84 ns | 0.6 ns | 0.182 ns |
S × α-toc | 3 | 3381.21 * | 548.0 ** | 1129.41 ns | 2793.45 ns | 0.79 ns | 3.51 ** | 2.2 * | 0.682 ns |
V × S × α-toc | 3 | 9211.3 *** | 9.3 ns | 404.06 ns | 3888.35 * | 0.96 ns | 0.60 ns | 0.2 ns | 0.182 ns |
Error | 48 | 1019.95 | 86.6 | 1014.53 | 1044.26 | 0.98 | 0.70 | 0.7 | 2.234 |
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Naqve, M.; Wang, X.; Shahbaz, M.; Fiaz, S.; Naqvi, W.; Naseer, M.; Mahmood, A.; Ali, H. Foliar Spray of Alpha-Tocopherol Modulates Antioxidant Potential of Okra Fruit under Salt Stress. Plants 2021, 10, 1382. https://doi.org/10.3390/plants10071382
Naqve M, Wang X, Shahbaz M, Fiaz S, Naqvi W, Naseer M, Mahmood A, Ali H. Foliar Spray of Alpha-Tocopherol Modulates Antioxidant Potential of Okra Fruit under Salt Stress. Plants. 2021; 10(7):1382. https://doi.org/10.3390/plants10071382
Chicago/Turabian StyleNaqve, Maria, Xiukang Wang, Muhammad Shahbaz, Sajid Fiaz, Wardah Naqvi, Mehwish Naseer, Athar Mahmood, and Habib Ali. 2021. "Foliar Spray of Alpha-Tocopherol Modulates Antioxidant Potential of Okra Fruit under Salt Stress" Plants 10, no. 7: 1382. https://doi.org/10.3390/plants10071382