Enhancing Resveratrol Bioproduction and Anti-Melanogenic Activities through Elicitation in DJ526 Cell Suspension
Abstract
:1. Introduction
2. Results and Discussion
2.1. Potential Elicitors of Resveratrol and Piceid Content in DJ526 Cell Suspension
2.1.1. Biotic Elicitors
2.1.2. Abiotic Elicitors
2.2. Elicitation Promotes Glycosyltransferase Activity
2.3. Role of MeJA in RS Gene Expression Relative to Total Resveratrol Production
2.4. Effect of DJ526 and MeJA-Treated Cell Suspension on Anti-Melanogenesis
3. Materials and Methods
3.1. Quantitation of Resveratrol and Piceid Content in Elicitor-Treated DJ526 Cell Suspension
3.1.1. Callus Induction
3.1.2. In Vitro Elicitor Treatment Preparations
3.1.3. Determination of Piceid and Resveratrol Content
3.1.4. Real-Time PCR for RS Gene and Glycosyltransferase Expression Analysis
3.2. In Vitro Skin Cell Culture
3.2.1. Cell Culture and Viability
3.2.2. Determination of Melanin Content and Tyrosinase Activity
3.2.3. Melanin Synthesis Gene Expression Using Real-Time PCR Analysis
3.3. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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No. | Gene | Gene ID | Description |
---|---|---|---|
1 | RS | AF227963 | stilbene synthase 3-like [Arachis hypogaea (peanut)] |
2 | QR1 | LOC4341323 | UDP-flavonoid 7-O-glucosyltransferase 73C6 (all organs) UDP-glycosyltransferase TURAN |
3 | QR2 | LOC4326220 | UDP-flavonoid 7-O-glucosyltransferase 73C6 (all organs) |
4 | QR3 | LOC4335169 | UDP-glycosyltransferase 74F2 (all organs) |
5 | QR4 | LOC43335168 | UDP-glycosyltransferase 74F2 (seeds) |
6 | QR5 | LOC4335166 | UDP-glycosyltransferase 74E2 (all organs) indole-3-acetate beta-glucosyltransferase |
7 | QR6 | LOC4334167 | UDP-glycosyltransferase 83A1 (all organs) |
8 | QR7 | LOC4333838 | UDP-glycosyltransferase 91B1 |
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Kantayos, V.; Kim, J.-S.; Baek, S.-H. Enhancing Resveratrol Bioproduction and Anti-Melanogenic Activities through Elicitation in DJ526 Cell Suspension. Plants 2021, 10, 1653. https://doi.org/10.3390/plants10081653
Kantayos V, Kim J-S, Baek S-H. Enhancing Resveratrol Bioproduction and Anti-Melanogenic Activities through Elicitation in DJ526 Cell Suspension. Plants. 2021; 10(8):1653. https://doi.org/10.3390/plants10081653
Chicago/Turabian StyleKantayos, Vipada, Jin-Suk Kim, and So-Hyeon Baek. 2021. "Enhancing Resveratrol Bioproduction and Anti-Melanogenic Activities through Elicitation in DJ526 Cell Suspension" Plants 10, no. 8: 1653. https://doi.org/10.3390/plants10081653