Influence of Schumann Range Electromagnetic Fields on Components of Plant Redox Metabolism in Wheat and Peas
Abstract
:1. Introduction
2. Results
Catalase and Superoxide Dismutase Activity
3. Discussion
4. Materials and Methods
4.1. Plant Growth and Magnetic Field Condition
4.2. Determination of MDA Content
4.3. Determination of Antioxidant Enzyme Activity
4.4. Superoxide Dismutase Isoforms Analysis
4.5. Catalase Isoforms Analysis
4.6. Thin Layer Chromatography
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Investigated Parameter | Object | Frequency | 7.8 Hz | 14.3 Hz | 20.8 Hz |
---|---|---|---|---|---|
SHORT-TIME | |||||
Catalase activity, µM H2O2/min×mg of protein | Wheat | control | 17.84 ± 2.05 | 15.89 ± 1.1 | 23.44 ± 1.37 |
MF | 16.24 ± 1.02 | 26.09 ± 1.38 * | 27.95 ± 2.49 * | ||
Pea | control | 24.32 ± 2.72 | 18.4 ± 3.63 | 26.65 ± 0.71 | |
MF | 25.73 ± 1.36 | 21.29 ± 1.89 | 23.61 ± 0.68 | ||
SOD activity, r.u/mg of protein | Wheat | control | 1.1 ± 0.41 | 0.61 ± 0.15 | 2.45 ± 0.51 |
MF | 0.81 ± 0.14 | 0.51 ± 0.11 | 1.92 ± 0.27 | ||
Pea | control | 0.42 ± 0.05 | 1.25 ± 0.11 | 1.84 ± 0.08 | |
MF | 0.44 ± 0.04 | 1.02 ± 0.14 | 1.87 ± 0.17 | ||
MDA, µM/g of dry weight | Wheat | control | 110.4 ± 1.11 | 136.78 ± 9.25 | 102.05 ± 1.2 |
MF | 123.11 ± 1.42 * | 147.27 ± 7.36 | 83.45 ± 1.31 * | ||
Pea | control | 235.87 ± 30.12 | 215.05 ± 9.42 | 177.68 ± 11.92 | |
MF | 275.83 ± 8.54 | 195.41 ± 13.92 | 227.91 ± 27.11 | ||
LONG-TIME | |||||
Catalase activity, µM H2O2/min×mg of protein | Wheat | control | 20.75 ± 1.03 | 19.24 ± 0.92 | 29.62 ± 1.69 |
MF | 19.85 ± 0.88 | 20.9 ± 1.06 | 33.8 ± 1.61 * | ||
Pea | control | 14.68 ± 0.42 | 22.07 ± 0.86 | 27.98 ± 0.74 | |
MF | 14.83 ± 0.51 | 17.56 ± 0.99 * | 26.73 ± 1.49 | ||
SOD activity, r.u/mg of protein | Wheat | control | 2.53 ± 0.36 | 5.24 ± 0.5 | 6.69 ± 0.86 |
MF | 3.19 ± 0.58 | 4.09 ± 0.25 | 4.59 ± 0.68 | ||
Pea | control | 5.77 ± 1.13 | 3.98 ± 0.55 | 6.99 ± 0.82 | |
MF | 3.3 ± 1.13 | 4.03 ± 0.72 | 12.47 ± 5.97 | ||
MDA, µM/g of dry weight | Wheat | control | 168.19 ± 12.26 | 133.75 ± 8.05 | 100.00 ± 5.67 |
MF | 182.69 ± 21.20 | 139.02 ± 6.55 | 104.06 ± 4.73 | ||
Pea | control | 224.10 ± 13.01 | 176.06 ± 16.38 | 271.11 ± 15.89 | |
MF | 231.65 ± 16.10 | 202.20 ± 17.85 | 260.46 ± 16.23 |
Object | Isoform | Control | 7.8 Hz | 14.3 Hz | 20.8 Hz |
---|---|---|---|---|---|
SHORT-TIME | |||||
Wheat | Mn-SOD | 13.6 ± 1.3 | 9.8 ± 0.3 | 7.3 ± 1.2 | 8.2 ± 0.9 |
Fe-SOD | 45.8 ± 0.2 | 46.7 ± 0.2 | 47.4 ± 1.3 | 46.6 ± 2.1 | |
SOD-3 | 43.5.0 ± 1.1 | 43.4 ± 0.4 | 45.2 ± 0.1 | 45.2 ± 3.0 | |
Pea | Mn-SOD | 26.7 ± 3.0 | 24.9 ± 2.4 | 23.7 ± 1.2 | 27.4 ± 2.0 |
Fe-SOD | 44.0 ± 2.6 | 44.2 ± 2.0 | 40.3 ± 3.2 | 38.8 ± 2.1 | |
SOD-3 | 29.3 ± 0.5 | 30.9 ± 4.4 | 36.0 ± 1.9 | 33.8 ± 0.1 | |
LONG-TIME | |||||
Wheat | Mn-SOD | 6.1 ± 1.1 | 8.8 ± 2.2 * | 5.2 ± 0.7 | 11.3 ± 1.2 * |
Fe-SOD | 41.1 ± 1.3 | 30.8 ± 4.7 | 38.3 ± 1.3 | 44.6 ± 3.5 | |
SOD-3 | 52.8 ± 1.2 | 60.3 ± 3.4 | 56.5 ± 1.5 | 44 ± 2.8 | |
Pea | Mn-SOD | 34.8 ± 3.2 | 27.7 ± 1.2 | 37.3 ± 3.6 | 39.1 ± 1.3 |
Fe-SOD | 34 ± 2.8 | 36.4 ± 1.6 | 36.5 ± 0.9 | 30.8 ± 1.4 | |
SOD-3 | 31.2 ± 1.2 | 35.9 ± 0.8 | 26.2 ± 2.8 | 30 ± 1.2 |
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Mshenskaya, N.; Sinitsyna, Y.; Kalyasova, E.; Valeria, K.; Zhirova, A.; Karpeeva, I.; Ilin, N. Influence of Schumann Range Electromagnetic Fields on Components of Plant Redox Metabolism in Wheat and Peas. Plants 2022, 11, 1955. https://doi.org/10.3390/plants11151955
Mshenskaya N, Sinitsyna Y, Kalyasova E, Valeria K, Zhirova A, Karpeeva I, Ilin N. Influence of Schumann Range Electromagnetic Fields on Components of Plant Redox Metabolism in Wheat and Peas. Plants. 2022; 11(15):1955. https://doi.org/10.3390/plants11151955
Chicago/Turabian StyleMshenskaya, Natalia, Yulia Sinitsyna, Ekaterina Kalyasova, Koshcheeva Valeria, Anastasia Zhirova, Irina Karpeeva, and Nikolay Ilin. 2022. "Influence of Schumann Range Electromagnetic Fields on Components of Plant Redox Metabolism in Wheat and Peas" Plants 11, no. 15: 1955. https://doi.org/10.3390/plants11151955