Molecular and Physiological Effects of Magnesium–Polyphenolic Compound as Biostimulant in Drought Stress Mitigation in Tomato
Abstract
:1. Introduction
2. Results
2.1. ALPAN® Treatment Preserved Photosynthesis in Tomato Seedlings under Water Deficit Condition
2.2. ALPAN® Treatment Alleviated Cell Damage and Protected Cellular Membranes in Tomato Seedlings under Water Deficit Condition
2.3. ALPAN® Treatment Modulated Proline Levels in Tomato Seedlings under Water Deficit Condition
2.4. ALPAN® Treatment Altered the Transcriptome in Tomato Seedlings under Water Deficit Condition
3. Discussion
4. Materials and Methods
4.1. Plant Material, Growing Conditions, and Experimental Setup
4.2. Chlorophyll and Carotenoids Quantification
4.3. Membrane Integrity Assessment by Conductivity of Electrolyte Leakage
4.4. Lipid Peroxidation by Quantification of Malondialdehyde Levels
4.5. Cellular Proline Quantification
4.6. RNA Isolation, cDNA Library Construction, and Gene Expression Analysis
4.7. RNA-Seq Analysis and Library Construction
4.8. Statistical Analysis
5. Patent
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Locus | Gene | Froward Primer | Reverse Primer | Product Size |
---|---|---|---|---|
Solyc01g108020 | SlEF1α | TGGAATTGGAACTGTCCCCG | GAGCTTCGTGGTGCATCTCT | 125 |
Solyc02g084850 | SlTAS14 | TCCCTACTCCCTGAACCTCC | CAGTCTTGCGCATTTGGTCT | 144 |
Solyc09g011850 | SlFLN1 | AGTGCAAACAAGGGCTGTCA | TCGGAGCTAAGCAGTGAATCC | 135 |
Solyc12g009400 | SlPDH1 | TGCTGCACGAGATCCTATCAG | TCATCCGCAAATTCGACAGC | 132 |
Solyc10g086180 | SlPAL6 | TTTCCAGGGCACTCCCATTG | GTCGTTGACAAGCTCGGAGA | 103 |
Solyc07g006500 | SlT6PS1 | GGTGTTTGCTCTGTTTATGGTGT | CAGAACAGTCATCAAGATTAAGCAG | 146 |
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Hamedeh, H.; Antoni, S.; Cocciaglia, L.; Ciccolini, V. Molecular and Physiological Effects of Magnesium–Polyphenolic Compound as Biostimulant in Drought Stress Mitigation in Tomato. Plants 2022, 11, 586. https://doi.org/10.3390/plants11050586
Hamedeh H, Antoni S, Cocciaglia L, Ciccolini V. Molecular and Physiological Effects of Magnesium–Polyphenolic Compound as Biostimulant in Drought Stress Mitigation in Tomato. Plants. 2022; 11(5):586. https://doi.org/10.3390/plants11050586
Chicago/Turabian StyleHamedeh, Haytham, Shaula Antoni, Lorenzo Cocciaglia, and Valentina Ciccolini. 2022. "Molecular and Physiological Effects of Magnesium–Polyphenolic Compound as Biostimulant in Drought Stress Mitigation in Tomato" Plants 11, no. 5: 586. https://doi.org/10.3390/plants11050586