Genome-Wide Identification of the MPK Gene Family and Expression Analysis under Low-Temperature Stress in the Banana
Abstract
:1. Introduction
2. Results
2.1. Identification of the MPK Gene Family in Bananas
2.2. Phylogenetic Analysis of the MPK Gene Family
2.3. Colinearity Analysis of the MPK Gene Family
2.4. KEGG Annotation Analysis of the MPK Gene Family
2.5. Prediction Analysis of the MPKs Promoter Cis-Acting Elements
2.6. Prediction of miRNA Regulation on MaMPKs
2.7. Expression Patterns of MPKs under Biotic and Abiotic Stress
2.8. The Expression Analysis of Group A under Low-Temperature Stress
3. Discussion
3.1. Interspecific Specificity and Genome Quality Together Determine the Number of MPKs in Bananas
3.2. MPKs Retain the Most Critical Functions during Evolution in the Banana
3.3. MaMPKs Are Negatively Regulated by miR172 in Low–Temperature Stress
4. Materials and Methods
4.1. Plant Materials
4.2. Identification of MPK Gene Family in Bananas
4.3. Protein Physicochemical Properties and Domain Analysis of MPKs
4.4. Phylogenetic Tree, Collinearity and Chromosome Localization Analysis
4.5. KEGG Annotation Analysis and Promoter Cis-Acting Element Prediction
4.6. Expression Analysis of MPKs and Prediction of miRNA
4.7. RNA Extraction and qRT-PCR
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fan, Z.; Zhao, B.; Lai, R.; Wu, H.; Jia, L.; Zhao, X.; Luo, J.; Huang, Y.; Chen, Y.; Lin, Y.; et al. Genome-Wide Identification of the MPK Gene Family and Expression Analysis under Low-Temperature Stress in the Banana. Plants 2023, 12, 2926. https://doi.org/10.3390/plants12162926
Fan Z, Zhao B, Lai R, Wu H, Jia L, Zhao X, Luo J, Huang Y, Chen Y, Lin Y, et al. Genome-Wide Identification of the MPK Gene Family and Expression Analysis under Low-Temperature Stress in the Banana. Plants. 2023; 12(16):2926. https://doi.org/10.3390/plants12162926
Chicago/Turabian StyleFan, Zhengyang, Bianbian Zhao, Ruilian Lai, Huan Wu, Liang Jia, Xiaobing Zhao, Jie Luo, Yuji Huang, Yukun Chen, Yuling Lin, and et al. 2023. "Genome-Wide Identification of the MPK Gene Family and Expression Analysis under Low-Temperature Stress in the Banana" Plants 12, no. 16: 2926. https://doi.org/10.3390/plants12162926